Luis Alejandro Jose Mur | Aberystwyth University - País de Gales

• Grant number: 16/50429-1
• Support Opportunities: Research Grants - Visiting Researcher Grant - International
• Start date: May 24, 2018
• End date: July 09, 2018
• Field of knowledge: Health Sciences - Medicine - Surgery
• Agreement: Newton Fund, with FAPESP as a partner institution in Brazil
• Principal Investigator: Patricia Pintor dos Reis
• Grantee: Patricia Pintor dos Reis
• Visiting researcher: Luis Alejandro Jose Mur
• Visiting researcher institution: Aberystwyth University, Wales
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

In this program, our main goals are: (Aim 1) To identify and validate microRNA (miRNA) expression in plasma and tumor tissue from patients, (Aim 2) To validate expression of predicted miRNA target genes in same samples, (Aim 3) To characterize metabolic changes in patient serum and sputum and integrate results with miRNA and target gene profiles. In order to achieve these goals, our research plan is to initially identify and validate biomarkers useful for early disease detection. In a second phase of our work, we will apply our scientific findings of miRNAs and metabolic changes to the characterization and validation of biomarkers with potential therapeutic application: 1) We will profile global miRNA expression in tumor and plasma samples from the same patients using the GeneChip® miRNA 4.0 Array (Affymetrix); 2) Data will be analyzed and interpreted in order to identify significantly deregulated miRNAs in tumor and plasma from patients; 3) Serum and sputum samples will be profile for metabolomic changes; 4) miRNA and metabolomic changes will be correlated; 5) Advanced bioinformatics and machine learning techniques will be applied in order to interpret changes in relation to regulatory pathways, disease development and progression; 6) Relevant clinical data will be analyzed in relation to identified changes. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE ARAUJO, RACHEL PAES; BERTONI, NATALIA; SENEDA, ANA L.; FELIX, TAINARA F.; CARVALHO, MARCIO; LEWIS, KEIR E.; HASIMOTO, ERICA N.; BECKMANN, MANFRED; DRIGO, SANDRA A.; REIS, PATRICIA P.; et al. Defining Metabolic Rewiring in Lung Squamous Cell Carcinoma. METABOLITES, v. 9, n. 3, MAR 7 2019. (16/50429-1)

SENEDA, ANA L.; LOPEZ LAPA, RAINER M.; FELIX, TAINARA F.; MINUTENTAG, IAEL W.; CAMPOS, CAROLINA F.; DE OLIVEIRA, ROGERIO A.; OLIVEIRA, CRISTIANO C.; HASIMOTO, ERICA N.; CATANEO, DANIELE C.; CATANEO, ANTONIO J. M.; et al. Typical Lung Carcinoids with Metastasis: Potential Role of MicroRNAs in the Regulation of Adaptive Immunity Associated with Disease: a Case Study. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, v. 55, p. 12-pg., 2021-01-01. (16/50429-1, 11/13213-7)