Multi-user equipment approved in grant 16/13368-4: spectrofluorimeter with polarizers

• Grant number: 17/04604-9
• Support Opportunities: Multi-user Equipment Program
• Start date: June 01, 2017
• End date: May 31, 2024
• Field of knowledge: Biological Sciences - Biophysics - Molecular Biophysics
• Principal Investigator: Karin Do Amaral Riske
• Grantee: Karin Do Amaral Riske
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated research grant: 16/13368-4 - Nanostructured systems: from membrane biomimetic models to carriers of bioactives, AP.TEM
• EMU web page: https://www.unifesp.br/reitoria/multiusuarios/equipamentos/paginas-dos-equipamentos/99-espectrofluorimetro-cary-eclipse-com-polarizadores-para-medidas-de-fluorescencia-e-anisotropia-de-fluorescencia
• Type of equipment: Caracterização de Materiais - Espectroscopia - Fluorescência (inclui resolvida no tempo)
• Manufacturer: Agilent
• Model: Cary Eclipse

Abstract

Fluorescence is one of the most versatile techniques applied in biophysical studies. The fluorescence lifetime is on the order of nanoseconds, coinciding with the timescale of several relevant processes at the molecular level, such as conformational changes of biomolecules. The fluorescence can be directly measured in biomolecules such as proteins and peptides that have fluorescent aminoacids, or specific fluorescent dyes can be used. Fluorescence anisotropy is a parameter obtained from a relation between the polarized light emitted and that of it excitation, and represents a measurement of the rotational movement of the fluorophore at the fluorescence timescale, indicating the microviscosity of the system of interest. (AU)