Compounds produced by endophyte microorganisms isolated from Brazilian tropical savannah: bioactivity, association with a-Ag2WO4 and biotechnological applications

• Grant number: 16/13423-5
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2017
• End date: September 30, 2019
• Field of knowledge: Biological Sciences - Biology
• Principal Investigator: Cristina Paiva de Sousa
• Grantee: Cristina Paiva de Sousa
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated researchers: Alberto Colli Badino Junior ; Antonio Gilberto Ferreira ; Fernando Lucas Primo ; Laécio Santos Cavalcante
• Associated scholarship(s): 17/12905-9 - Bioactive extracts produced by savanna endophytes for alpha-Ag2WO4 associated bioassays, BP.TT

Abstract

The Brazilian tropical savannah is a typical mosaic biome, characterized by endemic and medicinal plants with unique characteristics from where microorganisms can be isolated. Microorganisms are an important source for production of bioactive metabolites for diverse applications. Endophytes are microorganisms that inhabit the interior of plant tissues, without causing apparent disease to its host and they can produce bioactive substances. This project aims to answer three major questions like: a) can bioactive substances derived from endophytes be partially purified and act as antimicrobials? b) can these substances be used to reduce/eliminate chronic and fatal diseases like leishmaniasis? c) can nanoparticles be incorporated with the bioactive endophyte substances or extracts in order to present enhanced results? The importance of this project is linked to the crescent and progressively microbial resistance. In this sense, bioprospection can be used to search for new therapeutic substances that are produced by endophytic microorganisms alone or in association with nanoparticles. Tropical neglected diseases like Leishmaniasis could be treated with the discovery of new substances. Nanotechnology with antimicrobial metal synthesis can be used with synthesized biostatic and/or biocides particles like a-Ag2WO4 and similar. These particles in association with compounds produced by endophytes could enable biotechnological applications. (AU)

Scientific publications

(13)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALCOBACA, OLINDA S. A.; CAMPANINI, EMELINE B.; CIANCAGLINI, IARA; ROCHA, SAMARA, V; MALAVAZI, IRAN; FREIRE, CAIO C. M.; NUNES, FRANCIS M. F.; FUENTES, ANDREA S. C.; CUNHA, ANDERSON F.. Identification of a New Endo-beta-1,4-xylanase Prospected from the Microbiota of the Termite Heterotermes tenuis. MICROORGANISMS, v. 10, n. 5, p. 14-pg., 2022-05-01. (18/20823-5, 17/19694-3, 16/13423-5, 17/12905-9, 16/10130-7, 19/07505-7, 19/23964-1, 18/20697-0)

DE PAULA NOGUEIRA CRUZ, FELIPE; FERREIRA DE PAULA, AILTON; NOGUEIRA, CAMILA TITA; MARQUES DE ANDRADE, PAULO HENRIQUE; BORGES, LEONARDO MAURICI; LACAVA, PAULO TEIXEIRA; BARATELLA DA CUNHA CAMARGO, ILANA LOPES; ANIBAL, FERNANDA DE FREITAS; PAIVA DE SOUSA, CRISTINA. Discovery of a Novel Lineage Burkholderia cepacia ST 1870 Endophytically Isolated from Medicinal Polygala paniculata Which Shows Potent In Vitro Antileishmanial and Antimicrobial Effects. INTERNATIONAL JOURNAL OF MICROBIOLOGY, v. 2021, FEB 17 2021. (13/07600-3, 16/13423-5, 19/04788-8)

ASSIS, MARCELO; DA SILVA, JUSSARA SOARES; GONCALVES, MARIANA OTTAIANO; DE ALMEIDA RODOLPHO, JOICE MARGARETH; DE LIMA FRAGELLI, BRUNA DIAS; PEREIRA CORTE, ANA BEATRIZ; KELLY RIBEIRO, LARA; TEODORO, MARCIO DALDIN; ANIBAL, FERNANDA DE FREITAS; DE SOUSA, CRISTINA PAIVA; et al. Bactericidal activity of Ag4V2O7/beta-AgVO3 heterostructures against antibiotic-resistant Klebsiella pneumoniae. BIOMATERIALS ADVANCES, v. 141, p. 12-pg., 2022-09-05. (13/07296-2, 16/13423-5, 18/22214-6)

SERRANO, NADJA F. G.; FERREIRA, DOUGLAS; RODRIGUES-FILHO, EDSON; TEIXEIRA, JOSE ANTONIO; DUBREUIL, JEAN DANIEL; DE SOUSA, CRISTINA P.. Purification and characterization of two new antimicrobial molecules produced by an endophytic strain of Paenibacillus polymyxa. Anais da Academia Brasileira de Ciências, v. 93, n. 3, 2021. (16/13423-5)

MANDUJANO, GUSTAVO P. LORCA; ALVES, HENRIQUE C.; PRADO, CLEITON D.; MARTINS, JEFERSON G. O.; NOVAES, HOSANA R.; MAIA DE OLIVEIRA DA SILVA, JOAO PEDRO; TEIXEIRA, GLEIDSON SILVA; OHARA, ANDRE; ALVES, MATEUS H. R.; PEDRINO, ISADORA C.; et al. dentification and selection of a new Saccharomyces cerevisiae strain isolated from Brazilian ethanol fermentation process for application in beer productio. FOOD MICROBIOLOGY, v. 103, MAY 2022. (16/13423-5, 18/20697-0, 17/12905-9, 19/07505-7, 16/10130-7)

LIMA, M. C.; PAIVA DE SOUSA, C.; FERNANDEZ-PRADA, C.; HAREL, J.; DUBREUIL, J. D.; DE SOUZA, E. L.. A review of the current evidence of fruit phenolic compounds as potential antimicrobials against pathogenic bacteria. Microbial Pathogenesis, v. 130, p. 259-270, MAY 2019. (16/13423-5)

RIBEIRO, LARA K.; ASSIS, MARCELO; LIMA, LAIS R.; COELHO, DYOVANI; GONCALVES, MARIANA O.; PAIVA, ROBERT S.; MORAES, LEONARDO N.; ALMEIDA, LAUANA F.; LIPSKY, FELIPE; SAN-MIGUEL, MIGUEL A.; et al. Bioactive Ag3PO4/Polypropylene Composites for Inactivation of SARS-CoV-2 and Other Important Public Health Pathogens. Journal of Physical Chemistry B, v. 125, n. 38, p. 10866-10875, SEP 30 2021. (13/07296-2, 16/23891-6, 17/11986-5, 16/13423-5, 17/26105-4, 17/07711-0)

A. C. BOGAS; F. P. N. CRUZ; P. T. LACAVA; C. P. SOUSA. Fungos endofíticos: uma visão geral sobre o potencial biotecnológico e agronômico. Brazilian Journal of Biology, v. 84, 2022-06-08. (16/13423-5)

ASSIS, MARCELO; RIBEIRO, LARA K.; GONCALVES, MARIANA O.; STAFFA, LUCAS H.; PAIVA, ROBERT S.; LIMA, LAIS R.; COELHO, DYOVANI; ALMEIDA, LAUANA F.; MORAES, LEONARDO N.; ROSA, IEDA L. V.; et al. Polypropylene Modified with Ag-Based Semiconductors as a Potential Material against SARS-CoV-2 and Other Pathogens. ACS APPLIED POLYMER MATERIALS, v. 4, n. 10, p. 13-pg., 2022-09-16. (16/13423-5, 13/07296-2, 17/11986-5)

DA COSTA LIMA, M.; MAGNANI, M.; DOS SANTOS LIMA, M.; DE SOUSA, C. P.; DUBREUIL, J. D.; DE SOUZA, E. L.. Phenolic-rich extracts from acerola, cashew apple and mango by-products cause diverse inhibitory effects and cell damages on enterotoxigenic Escherichia coli. Letters in Applied Microbiology, NOV 2021. (16/13423-5)

BIANCHI, JAQUELINE; CAVICCHIOLI, RAFAEL; KUBOTA, LAURO T.; CARRILHO, EMANUEL; DE SOUSA, CRISTINA P.; FREITAS ANIBAL, FERNANDA DE. Antigenotoxic potential of the fermentation broth produced by Paenibacillus polymyxa RNC-D in vitro. FUTURE MICROBIOLOGY, v. 16, n. 7, p. 471-485, MAY 2021. (16/13423-5, 14/50867-3)

BOGAS, ANDREA CRISTINA; RODRIGUES, SAULO HENRIQUE; GONCALVES, MARIANA OTTAIANO; DE ASSIS, MARCELO; LONGO, ELSON; DE SOUSA, CRISTINA PAIVA. Endophytic Microorganisms From the Tropics as Biofactories for the Synthesis of Metal-Based Nanoparticles: Healthcare Applications. FRONTIERS IN NANOTECHNOLOGY, v. 4, p. 13-pg., 2022-02-24. (16/13423-5, 20/16299-9, 13/07296-2, 17/12905-9)

NERIS, DEBORA MEIRA; ORTOLANI, LETICIA GONCALVES; CASTRO, CYNTHIA APARECIDA DE; CORREIA, RICARDO DE OLIVEIRA; RODOLPHO, JOICE MARGARETH DE ALMEIDA; CAMILLO, LUCIANA; NOGUEIRA, CAMILA TITA; SOUSA, CRISTINA PAIVA DE; ANIBAL, FERNANDA DE FREITAS. In Vitro Modulator Effect of Total Extract from the Endophytic Paenibacillus polymyxa RNC-D in Leishmania (Leishmania) amazonensis and Macrophages. INTERNATIONAL JOURNAL OF MICROBIOLOGY, v. 2020, p. 11-pg., 2020-08-27. (16/13423-5, 14/07331-5)

Filed patent(s) as a result of this research project