Gastric cancer molecular and functional approaches in the Brazilian population

• Grant number: 16/25562-0
• Support Opportunities: Research Projects - Thematic Grants
• Start date: September 01, 2017
• End date: August 31, 2023
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Marilia de Arruda Cardoso Smith
• Grantee: Marilia de Arruda Cardoso Smith
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated researchers: Carolina de Oliveira Gigek ; Danielle Queiroz Calcagno ; Elizabeth Suchi Chen ; Fernanda Wisnieski ; Mariana Ferreira Leal ; Rommel Mario Rodríguez Burbano ; Spencer Luiz Marques Payão
• Associated scholarship(s): 21/08012-4 - Study of the expression of genes related to the telomere-telomerase complex in gastric Cancer, BP.IC; 20/00149-8 - Molecular and phenotypic evaluation of PCAF silecing in gastric cell lines, BP.TT; 19/20592-6 - Investigation of miRNAs differentialy expressed in plasma of patients with gastric adenocarcinoma., BP.PD; + associated scholarships 19/12334-7 - Validation of implicated miRNAs by qRT-PCR in Gastric Cancer samples, BP.TT; 18/26796-0 - Validation of implicated miRNAs by qRT-PCR in gastric cancer samples, BP.TT; 19/00077-0 - Molecular and phenotypical evaluation of PCAF silencing in gastric cell lines, BP.TT; 18/18522-7 - Analysis of 8q24.21 microRNAs expression in gastric carcinogenesis, BP.IC - associated scholarships

Abstract

Gastric cancer is the third leading cause of cancer death worldwide, besides the reduction in its incidence. Late diagnosis usually occurs due to the absence of symptoms or the presence of non-specific symptoms in early stages of the disease. In this case, the available therapeutic options are not efficient, resulting in high morbidity and mortality rates. The continuous study of new strategies for early diagnosis and identification of new therapeutic methods is of great interest in this disease. Our research group has been conducting studies on gastric cancer for over 15 years, in which different chromosomal, gene, protein, and epigenetic alterations have been identified. The purpose of this project is to deepen and extend our previous studies that have shown promising results, as well as, address new technologies for identifying possible biomarkers in gastric cancer. This project involves the analysis of transcriptional and genetic alterations in 8q24.21, validation of epigenetically modulated genes, molecular and phenotypic effects under overexpression and silencing of PCAF, BMP8B, MALAT1 and YWHAE genes in gastric cell lines, inhibition of MELK in cell lines treated with OTSSP167, the miRNoma investigation in gastric tissue samples and circulating signature of miRNAs and the genetic and epigenetic study of the telomere-telomerase complex. This study will allow a better understanding of etiology and physiopathology of gastric carcinogenesis and the identification of possible biomarkers, which may help in early diagnosis, prognosis and anticancer therapies monitoring. Additionally, these possible biomarkers may become targets in the development of new anticancer therapies. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ANAUATE, ANA CAROLINA; LEAL, MARIANA FERREIRA; QUEIROZ CALCAGNO, DANIELLE; GIGEK, CAROLINA OLIVEIRA; REAL KARIA, BRUNO TAKAO; WISNIESKI, FERNANDA; DOS SANTOS, LEONARDO CAIRES; CHEN, ELIZABETH SUCHI; BURBANO, ROMMEL RODRIGUEZ; CARDOSO SMITH, MARILIA ARRUDA. The Complex Network between MYC Oncogene and microRNAs in Gastric Cancer: An Overview. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 21, n. 5, MAR 2020. (16/19953-6, 16/25562-0, 19/20592-6, 12/14768-5)

REAL KARIA, BRUNO TAKAO; PINTO, CAMILA ALBUQUERQUE; CAIRES, LEONARDO; LOUREDO DOS SANTOS, YEDA BEATRIZ; WISNIESKI, FERNANDA; GIGEK, CAROLINA; CARDOSO SMITH, MARILIA DE ARRUDA. Investigation of differentially expressed miRNAs in patients with gastric adenocarcinoma. Cancer Research, v. 82, n. 12, p. 2-pg., 2022-06-15. (16/25562-0, 19/20592-6)

ALMEIDA, RENATA SANCHES; WISNIESKI, FERNANDA; REAL KARIA, BRUNO TAKAO; CARDOSO SMITH, MARILIA ARRUDA. CRISPR/Cas9 Genome-Editing Technology and Potential Clinical Application in Gastric Cancer. GENES, v. 13, n. 11, p. 13-pg., 2022-11-01. (16/25562-0, 19/20592-6)

SANTOS, M. P.; PEREIRA, J. N.; DELABIO, R. W.; SMITH, M. A. C.; PAYAO, S. L. M.; CARNEIRO, L. C.; BARBOSA, M. S.; RASMUSSEN, L. T.. Increased expression of interleukin-6 gene in gastritis and gastric cancer. Brazilian Journal of Medical and Biological Research, v. 54, n. 7, 2021. (16/25562-0, 18/02008-2, 18/08481-1)

PEREIRA, BRUNNO SANTOS; WISNIESKI, F. E. R. N. A. N. D. A.; CALCAGNO, DANIELLE QUEIROZ; SANTOS, LEONARDO CAIRES; GIGEK, CAROLINA OLIVEIRA; CHEN, ELIZABETH SUCHI; RASMUSSEN, LUCAS TREVIZANI; PAYAO, SPENCER LUIZ MARQUES; ALMEIDA, RENATA SANCHES; PINTO, CAMILA ALBUQUERQUE; et al. Genetic and Transcriptional Analysis of 8q24.21 Cluster in Gastric Cancer. ANTICANCER RESEARCH, v. 42, n. 9, p. 14-pg., 2022-09-01. (16/25562-0)

CALCAGNO, DANIELLE Q.; WISNIESKI, FERNANDA; DA SILVA MOTA, ELIZANGELA R.; MAIA DE SOUSA, STEFANIE B.; COSTA DA SILVA, JESSICA M.; LEAL, MARIANA F.; GIGEK, CAROLINA O.; SANTOS, LEONARDO C.; RASMUSSEN, LUCAS T.; ASSUMPCAO, PAULO P.; et al. Role of histone acetylation in gastric cancer: implications of dietetic compounds and clinical perspectives. Epigenomics, v. 11, n. 3, p. 349-362, FEB 2019. (16/25562-0, 12/14768-5, 16/19953-6)

SANTANA, DALILEIA APARECIDA; SMITH, MARILIA DE ARRUDA CARDOSO; CHEN, ELIZABETH SUCHI. Histone Modifications in Alzheimer's Disease. GENES, v. 14, n. 2, p. 18-pg., 2023-02-01. (16/25562-0)