Investigation of miRNAs differentialy expressed in plasma of patients with gastric adenocarcinoma.

• Grant number: 19/20592-6
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: November 01, 2019
• End date: August 31, 2023
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Marilia de Arruda Cardoso Smith
• Grantee: Bruno Takao Real Karia
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated research grant: 16/25562-0 - Gastric cancer molecular and functional approaches in the Brazilian population, AP.TEM

Abstract

Despite the reduction in worldwide incidence, gastric cancer remains the second leading cause of death caused by cancer. The delayed diagnosis of gastric cancer in general practice manly occurs due to the absence of symptoms or the presence of non-specific symptoms in the early stages of the disease. Consequently, only a few effective therapeutic options are available, resulting in high morbidity and mortality rates. The continuous study of new strategies for early diagnosis and identification of new therapeutic methods is of great interest in this neoplastic type. In this context, microRNAs (miRNAs) are a class of small RNA molecules involved in the post-transcriptional process of gene expression regulation. These molecules may also be used as biomarkers for early disease detection, since miRNAs aberrant expression occurs before the disease symptoms are observed. Moreover, miRNAs can be detected in body fluids, including plasma/serum. Therefore, the goals of this project are: (I): investigate miRNAs expression in the gastric tissue of tumor patients and in non-tumor patients and (II) use serum samples (circulating and exosome miRNAs), from tumor and non-tumor individuals, to validate the differentially expressed miRNAs initially found in gastric cancer tissue. Recent reports indicate that circulating miRNAs have great potential to be used as both a reliable biomarker and therapeutic target. Thus, is of great interest to elucidate its biological and oncological roles and to establish new miRNAs biomarkers in cancer patients. Additionally, biomarker miRNAs may have pharmacogenomic application and may be used as targets in the development of new antineoplastic therapies.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ANAUATE, ANA CAROLINA; LEAL, MARIANA FERREIRA; QUEIROZ CALCAGNO, DANIELLE; GIGEK, CAROLINA OLIVEIRA; REAL KARIA, BRUNO TAKAO; WISNIESKI, FERNANDA; DOS SANTOS, LEONARDO CAIRES; CHEN, ELIZABETH SUCHI; BURBANO, ROMMEL RODRIGUEZ; CARDOSO SMITH, MARILIA ARRUDA. The Complex Network between MYC Oncogene and microRNAs in Gastric Cancer: An Overview. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 21, n. 5, MAR 2020. (16/19953-6, 16/25562-0, 19/20592-6, 12/14768-5)

REAL KARIA, BRUNO TAKAO; PINTO, CAMILA ALBUQUERQUE; CAIRES, LEONARDO; LOUREDO DOS SANTOS, YEDA BEATRIZ; WISNIESKI, FERNANDA; GIGEK, CAROLINA; CARDOSO SMITH, MARILIA DE ARRUDA. Investigation of differentially expressed miRNAs in patients with gastric adenocarcinoma. Cancer Research, v. 82, n. 12, p. 2-pg., 2022-06-15. (16/25562-0, 19/20592-6)

ALMEIDA, RENATA SANCHES; WISNIESKI, FERNANDA; REAL KARIA, BRUNO TAKAO; CARDOSO SMITH, MARILIA ARRUDA. CRISPR/Cas9 Genome-Editing Technology and Potential Clinical Application in Gastric Cancer. GENES, v. 13, n. 11, p. 13-pg., 2022-11-01. (16/25562-0, 19/20592-6)