Development of tubular scaffolds for applications in vascular tissue engineering

• Grant number: 17/13273-6
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2017
• End date: April 30, 2020
• Field of knowledge: Engineering - Chemical Engineering - Chemical Technology
• Principal Investigator: Laís Pellizzer Gabriel
• Grantee: Laís Pellizzer Gabriel
• Host Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
• Associated researchers: Augusto Ducati Luchessi

Abstract

During the last decades, many attempts have been made to produce functional vascular substitutes that can be used to repair diseased blood vessels. However, currently available substitutes have presented inadequate mechanical, physiological or functional properties, leading to thrombosis or intimal hyperplasia following surgery. In this way, the objective of this study is to produce small-caliber (<6 mm) vascular substitutes using scaffolds to mimic the structure, morphology, mechanical and biological properties of the native blood vessels, having antithrombogenic properties and may hold the potential to repair blood vessels for vascular repair. In this way, scaffolds will be fabricated by rotary jet spinning polymer based on collagen, elastin and polyurethane. The influence of the fibers on the structure and morphology of the scaffolds will be evaluated using scanning electron microscopy and Fourier transform infrared spectroscopy. Thermal properties will be evaluated by thermogravimetry analysis. Mechanical properties will be evaluated using universal testing machine. Biological properties will be evaluated by blood platelet adhesion, cell adhesion, viability, proliferation and cell cycle. The biodegradation of the scaffolds will also be tested using in vitro mass loss. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RODRIGUES, ISABELLA C. P.; LOPES, EDER S. N.; PEREIRA, KARINA D.; HUBER, STEPHANY C.; JARDINI, ANDRE LUIZ; ANNICHINO-BIZZACCHI, JOYCE M.; LUCHESSI, AUGUSTO D.; GABRIEL, LAIS P.. Extracellular matrix-derived and low-cost proteins to improve polyurethane-based scaffolds for vascular grafts. SCIENTIFIC REPORTS, v. 12, n. 1, p. 12-pg., 2022-03-28. (20/07923-0, 17/13273-6)

RODRIGUES, ISABELLA CAROLINE PEREIRA; WOIGT, LUIZA FREIRE; PEREIRA, KARINA DANIELLE; LUCHESSI, AUGUSTO DUCATI; LOPES, EDER SOCRATES NAJAR; WEBSTER, THOMAS J.; GABRIEL, LAIS PELLIZZER. Low-cost hybrid scaffolds based on polyurethane and gelatin. JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T, v. 9, n. 4, p. 7777-7785, JUL-AUG 2020. (17/13273-6)

RODRIGUES, ISABELLA C. P.; PEREIRA, KARINA D.; WOIGT, LUIZA F.; JARDINI, ANDRE L.; LUCHESSI, AUGUSTO D.; LOPES, EDER S. N.; WEBSTER, THOMAS J.; GABRIEL, LAIS P.. A novel technique to produce tubular scaffolds based on collagen and elastin. ARTIFICIAL ORGANS, v. 45, n. 5, DEC 2020. (17/13273-6)

PEREIRA RODRIGUES, ISABELLA CAROLINE; TAMBORLIN, LETICIA; RODRIGUES, ANA AMELIA; JARDINI, ANDRE LUIZ; LUCHESSI, AUGUSTO DUCATI; MACIEL FILHO, RUBENS; NAJAR LOPES, EDER SOCRATES; GABRIEL, LAIS PELLIZZER. Polyurethane fibrous membranes tailored by rotary jet spinning for tissue engineering applications. Journal of Applied Polymer Science, SEP 2019. (17/13273-6)

PEREIRA RODRIGUES, ISABELLA CAROLINE; TAMBORLIN, LETICIA; RODRIGUES, ANA AMELIA; JARDINI, ANDRE LUIZ; LUCHESSI, AUGUSTO DUCATI; MACIEL FILHO, RUBENS; NAJAR LOPES, EDER SOCRATES; GABRIEL, LAIS PELLIZZER. Polyurethane fibrous membranes tailored by rotary jet spinning for tissue engineering applications. Journal of Applied Polymer Science, v. 137, n. 11, MAR 15 2020. (17/13273-6)