Characterization of bioactive compounds from annatto (Bixa orellana) seed oleaginous complex extracted via supercritical fluid and their effects on control of obesity and its comorbidities in vivo

• Grant number: 16/21480-9
• Support Opportunities: Research Grants - Innovative Research in Small Business - PIPE
• Start date: December 01, 2017
• End date: August 31, 2018
• Field of knowledge: Health Sciences - Nutrition - Nutrition Biochemistry
• Principal Investigator: Juliana Kelly da Silva Maia
• Grantee: Juliana Kelly da Silva Maia
• Company: Rubian Xtract Serviços Ltda
• City: Campinas
• Associated researchers: Marcio Lopes ; Mário Roberto Maróstica Junior
• Associated research grant(s): 20/05660-2 - The oleaginous complex of annatto seed (Bixa orellana) for application in weight control and comorbidities: product development, determination of efficacy and safety, AP.PIPE
• Associated scholarship(s): 17/26307-6 - Characterization of bioactive compounds from annatto (Bixa orellana) seed oleaginous complex extracted via supercritical fluid and their effects on control of Obesity and its comorbidities in vivo, BP.PIPE

Abstract

The present study will be carried out by Rubian Xtract Serviços Ltda, the company is incubated in the INOVA/UNICAMP and associated to business incubator from ESALQTEC/USP. Our aim is to characterize the bioactive compounds in the oleaginous complex, extracted from annatto seed (Bixa Orellana) via supercritical fluid process, their effects on obesity and its co-morbidities. The extraction will be carried out by green technique using Co2 in supercritical phase. The obtained oleaginous complex from annatto seed contains the bioactive of tocotrienol-geranylgeraniol, in addition to fatty acids. The above mentioned compounds will be determined by chromatographic techniques. Antioxidant capacity will be investigated using ORAClipophilic assay and the lipid peroxidation will be assessed by peroxide value tests and TBARS test. Citotoxicity will be evaluated in vitro by XTT method. Further, the biological potential will be assessed in an in vivo experiment of diet-induced obesity. Body weight, insulin resistance (GTT and ITT tests), lipid profile (total cholesterol and its fractions), inflammatory markers (cytokines), oxidative status (antioxidant enzymes), hepatic toxicity (ALT, AST and alkaline phosphatase) will be determined. Commercial products of tocopherol and tocotrienol will be used as control. According to previous results, the oleaginous complex from annatto seed from supercritical extraction may have synergic effect of tocotrienol and geranylgeraniol. Moreover, that complex may show a strong antioxidant, anti-inflammatory and hypocholesterolemic outcome, which acts on control of obesity and co-morbidities. Therefore, the product may be utilized on nutraceutical and nutracosmetics industries. (AU)

Filed patent(s) as a result of this research project