Development of immunochromatographic "dipstick" assay for direct detection of ranavirus

• Grant number: 16/21357-2
• Support type: Research Grants - Innovative Research in Small Business - PIPE
• Duration: December 01, 2017 - October 31, 2018
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal researcher: Sabrina Ribeiro de Almeida Queiroz
• Grantee: Sabrina Ribeiro de Almeida Queiroz
• Company: Dizoo Serviços de Atividades Veterinárias Eireli - ME
• City: Pirassununga
• Assoc. researchers: Niels Jørgen Olesen ; Ricardo Luiz Moro de Sousa ; Santiago Benites de Padua
• Associated scholarship(s): 18/13420-1 - Production of polyclonal antibodies for ranavirus detection, BP.TT; 17/25570-5 - Development of immunochromatographic dipstick assay for direct detection of ranavirus, BP.PIPE

Abstract

Aquaculture is seen as a strategic market for sustainable development, food production and expansion of unexplored frontiers in Brazil. However, the health aspects of production and the lack of infrastructure for the diagnosis of major infectious diseases are considered highly relevant barriers to increase production and productivity of herds in the different regions. The family Iridoviridae, especially ranaviruses, has been responsible for epidemics of great ecological and economic impacts on fish and amphibians of importance in aquaculture from various parts of the world, including Brazil. The diagnostic methods currently available, although they are very effective in detection of ranaviruses, require specialized training and equipment for proper performance and the results are not immediate. Thus, the aim of this project is to develop a direct, sensitive and specific immunochromatographic test for rapid and qualitative detection of ranavirus in samples of fish and amphibians. Therefore, the methodology will consist of three stages, consisting of interdependent experiments: the first experiment will comprise the gene expression of MCP protein of ranavirus (FV3-like); the second experiment will comprise the production of anti-MCP polyclonal antibody in rabbits; and the third experiment will comprise the assembly and validation of the immunochromatographic dipstick test for ranavirus detection. It is expected that a rapid, sensitive and feasible test diagnostic for field conditions can collaborate for a better understanding of the movement of such infectious agents in fish and amphibians from Brazil and thus may promote measures for their control and prevention of ranavirus infection. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CANDIDO, MARCELO; TAVARES, LOIANE SAMPAIO; ALENCAR, ANNA LUIZA FARIAS; FERREIRA, CLAUDIA MARIS; DE ALMEIDA QUEIROZ, SABRINA RIBEIRO; FERNANDES, ANDREZZA MARIA; MORO DE SOUSA, RICARDO LUIZ. Genome analysis of Ranavirus frog virus 3 isolated from American Bullfrog (Lithobates catesbeianus) in South America. SCIENTIFIC REPORTS, v. 9, NOV 20 2019. Web of Science Citations: 0.