Genomic analysis of high-risk clones of Acinetobacter baumannii from different hospitals in Brazil

• Grant number: 17/16988-6
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2018
• End date: February 29, 2020
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Carlos Henrique Camargo
• Grantee: Carlos Henrique Camargo
• Host Institution: Instituto Adolfo Lutz (IAL). Coordenadoria de Controle de Doenças (CCD). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Associated researchers: Enéas de Carvalho ; Erica Chimara Silva ; Monique Ribeiro Tiba Casas

Abstract

The apocalyptical post-antimicrobial era has already dawned. Pan-resistant pathogens that require the use of combined therapy are already circulating in several hospitals around the world. As an attempt to reduce the spread of resistant bacteria, the United Nations proposed a combined plan to fight the antimicrobial resistance. One of the most worrisome resistant pathogen currently spread in hospitals is carbapenem-resistant Acinetobacter baumannii (CRAB). These pathogens has the ability to acquire several resistance mechanisms, and to persist in the hospital settings over time. In Brazil, a country with high endemics rates of CRAB, molecular epidemiology revealed the occurrence of four major clonal complexes, namely CC1, CC15, CC25 and CC79, known as "high-risk clones". The reasons to explain the success of these high-risk clones CRAB are not well elucidated so far, but genomic analysis can explain micro evolutionary aspects of their establishment in Brazil. By using large-scale and deep sequencing, the highest discriminatory level of identification of bacterial species can be achieved. The Next-Generation sequencing NGS (Illumina platforms) will be employed to characterize the resistome, mobilome and plasmidome of 96 Acinetobacter strains recovered from 30 different Brazilian institutions. Correlations between the antimicrobial susceptibility testing and the NGS results will be done, in order to provide an in silico approach to detect antimicrobial resistance. The MALDI-TOF technology will also be employed to find presumptive markers of the high-risk clones A. baumannii. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BRISOLLA TAVARES, LAIS CALISSI; VIEIRA CUNHA, MARCOS PAULO; DE VASCONCELLOS, FRANCIELLI MAHNIC; DE JESUS BERTANI, AMANDA MARIA; FRANCO DE BARCELLOS, THAYS ALMEIDA; BUENO, MARIANA SARDINHA; SANTOS, CARLA ADRIANA; SANT'ANA, DANIEL ALMEIDA; FERREIRA, ADRIANO MARTISON; MONDELLI, ALESSANDRO LIA; et al. Genomic and Clinical Characterization of IMP-1-Producing Multidrug-Resistant Acinetobacter bereziniae Isolates from Bloodstream Infections in a Brazilian Tertiary Hospital. MICROBIAL DRUG RESISTANCE, v. 26, n. 11, MAR 2020. (16/20727-0, 15/13179-4, 17/16988-6)

DE BARCELLOS, THAYS ALMEIDA FRANCO; BUENO, MARIANA SARDINHA; CUNHA, MARCOS PAULO VIEIRA; NAGAMORI, FILIPE ONISHI; DE CARVALHO, ENEAS; TAKAGI, ELIZABETH HARUMMYY; MORENO, LUISA ZANOLLI; MORENO, ANDREA MICKE; CHIMARA, ERICA; TIBA-CASAS, MONIQUE RIBEIRO; et al. Silent mutations in ribosomal protein genes are associated with high-risk clones of carbapenem-resistant Acinetobacter baumannii prevalent in Brazil. INFECTION GENETICS AND EVOLUTION, v. 88, MAR 2021. (17/16988-6)

BUENO, MARIANA SARDINHA; FREIRE, MARISTELA PINHEIRO; VIEIRA CUNHA, MARCOS PAULO; FRANCO DE BARCELLOS, THAYS ALMEIDA; DE JESUS BERTANI, AMANDA MARIA; DOS SANTOS, CARLA ADRIANA; CHIMARA, ERICA; NAGAMORI, FILIPE ONISHI; TAKAGI, ELIZABETH HARUMMYY; COSTA, SILVIA FIGUEIREDO; et al. Detection of pandrug-resistant ST15 Acinetobacter baumannii causing bloodstream infection in an HSCT patient in Brazil. Journal of Antimicrobial Chemotherapy, v. 75, n. 9, p. 4-pg., 2020-09-01. (17/16988-6)

CAMARGO, CARLOS HENRIQUE; YAMADA, AMANDA YAEKO; NAGAMORI, FILIPE ONISHI; DE SOUZA, ANDREIA RODRIGUES; TIBA-CASAS, MONIQUE RIBEIRO; DE MORAES FRANCA, FLAVIA APARECIDA; TRIGUEIRO NOBREGA PORTO, MARIA HELENA; DE LIMA GARZON, MAGDA LOURDES; HIGGINS, PAUL; MADALOSSO, GERALDINE; et al. Clonal spread of ArmA- and OXA-23-coproducing Acinetobacter baumannii International Clone 2 in Brazil during the first wave of the COVID-19 pandemic. Journal of Medical Microbiology, v. 71, n. 4, p. 6-pg., 2022-01-01. (17/16988-6, 20/06157-2, 17/50333-7, 18/21192-9, 18/21193-5)