A wide genome association study for resistance to Fusarium oxysporum f. sp. phaseoli and for tegument color in common bean

• Grant number: 17/24711-4
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2018
• End date: August 31, 2020
• Field of knowledge: Agronomical Sciences - Agronomy - Crop Science
• Principal Investigator: Luciana Lasry Benchimol-Reis
• Grantee: Luciana Lasry Benchimol-Reis
• Host Institution: Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Campinas , SP, Brazil
• City of the host institution: Campinas
• Associated researchers: Alisson Fernando Chiorato ; Antonio Augusto Franco Garcia ; Luis Eduardo Aranha Camargo ; Renata Oliveira Batista
• Associated scholarship(s): 18/08582-2 - A wide genome association study for resistance to Fusarium oxysporum f. SP. phaseoli and for tegument color in common bean, BP.TT

Abstract

Fusarium wilt (Fop) stands out as being the main soil disease in bean crop, with a large occurrence worldwide, can cause losses of up to 100% in productivity in extreme cases. The most economical and efficient method for its control is the use of resistant cultivars, but the physiological variability of the pathogen makes difficult the development of resistant cultivars by breeding programs. Genome wide association studies (GWAS) allow the identification of loci that influence the expression of quantitative characteristics. In this context, the objective of this work will be to use a set of 6,000 SNPs (BARCBean6K_3 BeadChip) in 288 accessions of the IAC germplasm bank (BAG) to identify genomic regions associated with Fop resistance genes, grain staining, and browning of grains. Significant markers will be located, and chromosomes with the highest number of significant SNPs explain the highest proportion of variation in Fop resistance and tegument color, and indicate whether there are any genes and / or QTLs associated and in which regions of the genome. Accelerated grain darkening for 'carioca' type and Nuclear Magnetic Resonance analysis with all panel genotypes will be performed. For resistance to fusarium wilt, two isolates of Brazilian breed 2, characterized by UFV (Viçosa, MG) as virulent, will be used. Bulks containing 60 genotypes of clear tegument and 60 genotypes of dark tegument of the 'carioca' type will be analyzed with microsatellites in the search of polymorphic markers for selection assisted by markers. When polymorphic, these markers will be analyzed in all Bulk genotypes and will be incorporated into associative mapping. This study is expected to provide genetic evidence of the relationship between grain staining, browning and Fop resistance, providing tools to achieve lasting resistance to this disease. (AU)

Scientific publications

(10)

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

JEAN FAUSTO DE CARVALHO PAULINO; CALÉO PANHOCA DE ALMEIDA; GABRIEL DE MORAES CUNHA GONÇALVES; CÉSAR JÚNIOR BUENO; SÉRGIO AUGUSTO MORAIS CARBONELL; ALISSON FERNANDO CHIORATO; LUCIANA LASRY BECHIMOL-REIS. Assessment of resistance in common bean to Fusarium oxysporum f. sp. phaseoli using different inoculation and evaluation methods. Crop Breeding and Applied Biotechnology, v. 20, n. 3, 2020-10-16. (17/24711-4)

DE CARVALHO PAULINO, JEAN FAUSTO; DE ALMEIDA, CALEO PANHOCA; SONG, QIJIAN; CARBONELL, SERGIO AUGUSTO MORAIS; CHIORATO, ALISSON FERNANDO; BENCHIMOL-REIS, LUCIANA LASRY. Genetic diversity and inter-gene pool introgression of Mesoamerican Diversity Panel in common beans. JOURNAL OF APPLIED GENETICS, v. 62, n. 4, AUG 2021. (19/19670-2, 18/15526-1, 17/01753-3, 17/24711-4)

ALMEIDA, CALEO PANHOCA DE; DE CARVALHO PAULINO, JEAN FAUSTO; BONFANTE, GABRIEL FRANCESCO JANINI; PERSEGUINI, JULIANA MORINI KUPPER CARDOSO; SANTOS, ISABELLA LAPORTE; GONCALVES, JOAO GUILHERME RIBEIRO; PATRICIO, FLAVIA RODRIGUES ALVES; TANIGUTI, CRISTIANE HAYUMI; GESTEIRA, GABRIEL DE SIQUEIRA; GARCIA, ANTONIO AUGUSTO FRANCO; et al. Angular Leaf Spot Resistance Loci Associated With Different Plant Growth Stages in Common Bean. FRONTIERS IN PLANT SCIENCE, v. 12, APR 13 2021. (18/20992-1, 18/15526-1, 19/19670-2, 14/11145-2, 17/24711-4, 17/01753-3, 14/08280-5)

DE CARVALHO PAULINO, JEAN FAUSTO; DE ALMEIDA, CALEO PANHOCA; CUNHA GONCALVES, GABRIEL DE MORAES; BUENO, CESAR JUNIOR; MORAIS CARBONELL, SERGIO AUGUSTO; CHIORATO, ALISSON FERNANDO; BECHIMOL-REIS, LUCIANA LASRY. Assessment of resistance in common bean to Fusarium oxysporum f. sp. phaseoli using different inoculation and evaluation methods. Crop Breeding and Applied Biotechnology, v. 20, n. 3, p. 6-pg., 2020-01-01. (17/24711-4)

ALMEIDA, CALEO PANHOCA DE; PAULINO, JEAN FAUSTO DE CARVALHO; MORAIS CARBONELL, SERGIO AUGUSTO; CHIORATO, ALISSON FERNANDO; SONG, QIJIAN; DI VITTORI, VALERIO; RODRIGUEZ, MONICA; PAPA, ROBERTO; BENCHIMOL-REIS, LUCIANA LASRY. Genetic Diversity, Population Structure, and Andean Introgression in Brazilian Common Bean Cultivars after Half a Century of Genetic Breeding. GENES, v. 11, n. 11, NOV 2020. (17/01753-3, 17/24711-4, 19/19670-2, 18/15526-1)

DE CARVALHO PAULINO, JEAN FAUSTO; DE ALMEIDA, CALEO PANHOCA; BUENO, CESAR JUNIOR; SONG, QIJIAN; FRITSCHE-NETO, ROBERTO; MORAIS CARBONELL, SERGIO AUGUSTO; CHIORATO, ALISSON FERNANDO; BENCHIMOL-REIS, LUCIANA LASRY. Genome-Wide Association Study Reveals Genomic Regions Associated with Fusarium Wilt Resistance in Common Bean. GENES, v. 12, n. 5, MAY 2021. (19/19670-2, 18/15526-1, 17/01753-3, 17/24711-4)

DE ALMEIDA, CALEO PANHOCA; DE CARVALHO PAULINO, JEAN FAUSTO; FERRARI BARBOSA, CAIO CESAR; CUNHA GONCALVES, GABRIEL DE MORAES; FRITSCHE-NETO, ROBERTO; MORAIS CARBONELL, SERGIO AUGUSTO; CHIORATO, ALISSON FERNANDO; BENCHIMOL-REIS, LUCIANA LASRY. Genome-wide association mapping reveals race-specific SNP markers associated with anthracnose resistance in carioca common beans. PLoS One, v. 16, n. 5, MAY 19 2021. (17/24711-4, 19/19670-2)

DE ALMEIDA, CALEO PANHOCA; SANTOS, ISABELLA LAPORTE; DE CARVALHO PAULINO, JEAN FAUSTO; FERRARI BARBOSA, CAIO CESAR; AUGUSTO PEREIRA, CASSIA CRISTINA; LIMONTA CARVALHO, CASSIA REGINA; CUNHA GONCALVES, GABRIEL DE MORAES; SONG, QIJIAN; MORAIS CARBONELL, SERGIO AUGUSTO; CHIORATO, ALISSON FERNANDO; et al. Genome-wide association mapping reveals new loci associated with light-colored seed coat at harvest and slow darkening in carioca beans. BMC PLANT BIOLOGY, v. 21, n. 1, JUL 20 2021. (18/20992-1, 17/24711-4, 19/19670-2)

DE CARVALHO PAULINO, JEAN FAUSTO; DE ALMEIDA, CALEO PANHOCA; FERRARI BARBOSA, CAIO CESAR; DE MORAES, GABRIEL; GONCALVES, CUNHA; BUENO, CESAR JUNIOR; HARAKAVA, RICARDO; MORAIS CARBONELL, SERGIO AUGUSTO; CHIORATO, ALISSON FERNANDO; BENCHIMOL-REIS, LUCIANA LASRY. Molecular and pathogenicity characterization of Fusarium oxysporum species complex associated with Fusarium wilt of common bean in Brazil. ROPICAL PLANT PATHOLOG, v. N/A, p. 10-pg., 2022-03-16. (17/24711-4, 17/01753-3, 18/15526-1, 19/19670-2)

SALVADOR, MARILIA VILELA; CRIZOSTOMO KOCK, FLAVIO VINICIUS; SANTOS, ISABELLA LAPORTE; CARVALHO PAULINO, JEAN FAUSTO; DE ALMEIDA, CALEO PANHOCA; DOS SANTOS GARCIA, RODRIGO HENRIQUE; BENCHIMOL-REIS, LUCIANA LASRY; COLNAGO, LUIZ ALBERTO; FERREIRA, ANTONIO GILBERTO. Monitoring Stimulated Darkening from UV-C Light on Different Bean Genotypes by NMR Spectroscopy. Molecules, v. 27, n. 7, p. 10-pg., 2022-04-01. (21/12694-3, 18/16040-5, 18/08582-2, 17/24711-4)