Organoid-like intestinal tridimensional (3D) culture for studies on virulence and/or probiotic properties of bacteria and food related compounds

• Grant number: 17/18928-0
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2018
• End date: March 31, 2020
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Science
• Principal Investigator: Elaine Cristina Pereira de Martinis
• Grantee: Elaine Cristina Pereira de Martinis
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant(s): 20/00898-0 - High-throughput DNA sequencing combined with tridimensional eukaryotic cell culture applied to the study of bacterial pathogenesis: the case of Methicillin-Resistant Staphylococcus aureus, AP.R SPRINT

Abstract

Currently, applications of eukaryotic cell cultures are being developed in three-dimensional (3D) systems, which renders organotypic structures that mimics what occurs in vivo. In the case of intestinal cell culture model, it is possible to obtain a highly differentiated three-dimensional structure, which can be very useful for studies of mechanisms of interaction with pathogenic and/or probiotic bacteria, as well as compounds of interest in food.In this project, a model of Caco-2 intestinal cell culture with a Slow Turning Lateral Device, will be started in the Laboratory of Food Microbiology - FCFRP-USP for applications in studies of global gene expression (transcriptomic) to evaluate possible mechanisms of action of pathogenic cultures, probiotics and/or bacteriocins.This proposal is related to a project developed abroad by the applicant with FAPESP support (2016 / 16293-5) at the University of Pennsylvania, USA (January to July 2017) and will promote the dissemination of new knowledge in the institution of origin (FCFRP- USP), as well as strengthen national and international collaborations (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEREIRA, MARITA GIMENEZ; DE ALMEIDA, OTAVIO GUILHERME GONCALVES; DA SILVA, HEVELIN REGIANE AUGUSTO; ISHIZAWA, MARILIA HARUMI; DE MARTINIS, ELAINE CRISTINA PEREIRA. Studies on host-foodborne bacteria in intestinal three-dimensional cell culture model indicate possible mechanisms of interaction. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, v. 37, n. 2, FEB 2021. (17/18928-0, 17/13759-6, 16/16293-5)

REIS-TEIXEIRA, FERNANDA BARBOSA; SOUSA, INGRID PONTES; ALVES, VIRGINIA FARIAS; JACOMETTI CARDOSO FURTADO, NIEGE ARACARI; PEREIRA DE MARTINIS, ELAINE CRISTINA. Evaluation of lemongrass and ginger essential oils to inhibit Listeria monocytogenes in biofilms. JOURNAL OF FOOD SAFETY, v. 39, n. 4, AUG 2019. (10/10051-3, 17/18928-0, 11/07062-6)