Evaluation of the efficiency of lactic acid bacteria and Saccharomyces cerevisiae in reducing the bioavailability of aflatoxin M1 in Minas frescal cheese

• Grant number: 17/20081-6
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2018
• End date: March 31, 2020
• Field of knowledge: Agronomical Sciences - Food Science and Technology
• Principal Investigator: Carlos Humberto Corassin
• Grantee: Carlos Humberto Corassin
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil

Abstract

The project aims to verify the occurrence of aflatoxin M1 in milk samples and 'Minas frescal' cheese from dairies (monitored by Brazilian agriculture ministry), which yield this dairy product with and without the addition of lactic acid bacteria. Additionally, the specific aim of the proposed study will be to evaluate the efficiency of lactic acid bacteria and Saccharomyces cerevisiae for use as food additive to reduce the bioavailability of aflatoxin M1 in Minas frescal cheese. Fresh Minas cheeses will be experimentally produced with aflatoxin M1 contaminated milk, and lactic acid bacteria and Saccharomyces cerevisiae will be added during the cheese production process for testing the bioavailability reduction. The efficiency of these microorganisms in reducing the bioavailability of aflatoxin M1 in Minas frescal cheese will be evaluated through the high-performance liquid chromatography (HPLC) and the possible effects on the shelf life will be evaluated through physical-chemical and microbiological analyses. The identification of the binding mechanism between lactic acid bacteria, Saccharomyces cerevisiae and aflatoxin M1 will be performed by usage of the scanning electron microscopy. Statistical analyzes will be performed in accordance with the procedures established in the SAS General Linear Model to observe significant differences. It is expected with the present study to determine the effect of the addition of these microorganisms on the shelf life of Minas frescal cheese, and to identify the binding mechanism associated with lactic acid bacteria, Saccharomyces cerevisiae and aflatoxin M1. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GONCALVES, BRUNA L.; ULLIANA, ROMULO D.; RAMOS, GUSTAVO L. P. A.; CRUZ, ADRIANO G.; OLIVEIRA, CARLOS A. F.; KAMIMURA, ELIANA S.; CORASSIN, CARLOS H.. Occurrence of aflatoxin M-1 in milk and Minas Frescal cheese manufactured in Brazilian dairy plants. INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY, v. 74, n. 2, MAR 2021. (17/19683-1, 17/20081-6)

BRUNA LEONEL GONÇALVES; ROMULO DUTRA ULIANA; SARAH HWA LEE; CAROLINA FERNANDA COPPA; CARLOS AUGUSTO FERNANDES DE OLIVEIRA; ELIANA SETSUKO KAMIMURA; CARLOS HUMBERTO CORASSIN. Use of scanning electron microscopy and high-performance liquid chromatography to assess the ability of microorganisms to bind aflatoxin M1 in Minas Frescal cheese. FOOD SCIENCE AND TECHNOLOGY, v. 42, 2021-02-01. (17/20081-6, 17/19683-1)

GONCALVES, BRUNA LEONEL; MUAZ, KHURRAM; SENGLING CEBIN COPPA, CAROLINA FERNANDA; ROSIM, ROICE ELIANA; KAMIMURA, ELIANA SETSUKO; FERNANDES OLIVEIRA, CARLOS AUGUSTO; CORASSIN, CARLOS HUMBERTO. Aflatoxin M-1 absorption by non-viable cells of lactic acid bacteria and Saccharomyces cerevisiae strains in Frescal cheese. Food Research International, v. 136, OCT 2020. (17/20081-6, 17/19683-1)

MOLLER, CLEIDE OLIVEIRA DE ALMEIDA; FREIRE, LUISA; ROSIM, ROICE ELIANA; MARGALHO, LARISSA PEREIRA; BALTHAZAR, CELSO FASURA; FRANCO, LARISSA TUANNY; SANT'ANA, ANDERSON DE SOUZA; CORASSIN, CARLOS HUMBERTO; RATTRAY, FERGAL PATRICK; OLIVEIRA, CARLOS AUGUSTO FERNANDES DE. Effect of Lactic Acid Bacteria Strains on the Growth and Aflatoxin Production Potential of Aspergillus parasiticus, and Their Ability to Bind Aflatoxin B-1, Ochratoxin A, and Zearalenone in vitro. FRONTIERS IN MICROBIOLOGY, v. 12, APR 22 2021. (17/20081-6, 17/03899-5, 15/25641-4, 16/21041-5, 18/24540-8)

GONCALVES, BRUNA LEONEL; ULIANA, ROMULO DUTRA; COPPA, CAROLINA FERNANDA SENGLING CEBIN; LEE, SARAH HWA IN; KAMIMURA, ELIANA SETSUKO; OLIVEIRA, CARLOS AUGUSTO FERNANDES; CORASSIN, CARLOS HUMBERTO. Aflatoxin M-1: biological decontamination methods in milk and cheese. FOOD SCIENCE AND TECHNOLOGY, v. 42, p. 8-pg., 2022-01-01. (17/19683-1, 17/20081-6)

PIRES, ROGERIO C.; PORTINARI, MARINA R. P.; MORAES, GIULIA Z.; KHANEGHAH, AMIN MOUSAVI; GONCALVES, BRUNA L.; ROSIM, ROICE E.; OLIVEIRA, CARLOS A. F.; CORASSIN, CARLOS H.. Evaluation of Anti-Aflatoxin M-1 effects of heat-killed cells of Saccharomyces cerevisiae in Brazilian commercial yogurts. QUALITY ASSURANCE AND SAFETY OF CROPS & FOODS, v. 14, n. 1, p. 7-pg., 2022-01-01. (17/20081-6)

BRUNA LEONEL GONÇALVES; ROMULO DUTRA ULIANA; CAROLINA FERNANDA SENGLING CEBIN COPPA; SARAH HWA IN LEE; ELIANA SETSUKO KAMIMURA; CARLOS AUGUSTO FERNANDES OLIVEIRA; CARLOS HUMBERTO CORASSIN. Aflatoxin M1: biological decontamination methods in milk and cheese. FOOD SCIENCE AND TECHNOLOGY, v. 42, 2020-10-09. (17/20081-6, 17/19683-1)

GONCALVES, BRUNA LEONEL; ULIANA, ROMULO DUTRA; LEE, SARAH HWA; COPPA, CAROLINA FERNANDA; FERNANDES DE OLIVEIRA, CARLOS AUGUSTO; KAMIMURA, ELIANA SETSUKO; CORASSIN, CARLOS HUMBERTO. Use of scanning electron microscopy and high-performance liquid chromatography to assess the ability of microorganisms to bind aflatoxin M-1 in Minas Frescal cheese. FOOD SCIENCE AND TECHNOLOGY, v. 42, p. 8-pg., 2022-01-01. (17/20081-6)

CAMPAGNOLLO, FERNANDA B.; KHANEGHAH, AMIN MOUSAVI; BORGES, LILIANA L.; BONATO, MELINA A.; FAKHRI, YADOLAH; BARBALHO, CAIO B.; BARBALHO, RICARDO L. C.; CORASSIN, CARLOS H.; OLIVEIRA, CARLOS A. F.. In vitro and in vivo capacity of yeast-based products to bind to aflatoxins B-1 and M-1 in media and foodstuffs: A systematic review and meta-analysis. Food Research International, v. 137, NOV 2020. (17/20081-6)

BRUNA LEONEL GONÇALVES; ROMULO DUTRA ULIANA; SARAH HWA LEE; CAROLINA FERNANDA COPPA; CARLOS AUGUSTO FERNANDES DE OLIVEIRA; ELIANA SETSUKO KAMIMURA; CARLOS HUMBERTO CORASSIN. Use of scanning electron microscopy and high-performance liquid chromatography to assess the ability of microorganisms to bind aflatoxin M1 in Minas Frescal cheese. FOOD SCIENCE AND TECHNOLOGY, n. ahead, p. -, 2021. (17/19683-1, 17/20081-6)