Development and characterization of a bienzymatic biosensor to determine sugars in dietetic food

• Grant number: 05/00294-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: October 01, 2005
• End date: February 28, 2009
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Sergio Antonio Spinola Machado
• Grantee: Andressa Galli
• Host Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil

Abstract

This project aim to develop, characterize and apply a bienzimatic biosensors prepared by modifying a polycrystalline Au electrode with a self-assembly monolayer of mercaptopropionic acid and mercaptoundecanoic acid. This monolayer will be further modified by glucose oxidase, fructose dehydrogenase enzymes and tetrathiafulvalene adsorption, for the electrochemical determination of sugars (glucose and fructose) in dietetic foods. The determinations using the enzymes modified electrodes will be made by chronoamperometric method, using the electrochemical oxidation of an espeficic mediator (tetrathiafulvalene for glucose oxidase and 3,4-dihydroxibenzaldehide for the fructose dehydrogenase enzymes, respectively) whose oxidation state is changed by the individual action of the enzymatic in its specific substrate. The electrochemical determinations (by square wave voltammetric) of the mediators will yield an electroanalytical method to the used for sugar determination in dietetic foods. The influence of some possible contaminants in these matrices will be also analyzed. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DJENAINE DE SOUZA; ANDRESSA GALLI; MARCELO L. CALEGARO; SERGIO A. S. MACHADO; ROBERTO C. PIRES. Utilização da múltipla voltametria de onda quadrada na determinação eletroanalítica de compostos orgânicos e inorgânicos. Química Nova, v. 30, n. 2, p. 458-463, 2007-04-00. (05/00294-8)

GALLI, ANDRESSA; DE SOUZA, DJENAINE; MACHADO, SERGIO A. S.. Pendimethalin determination in natural water, baby food and river sediment samples using electroanalytical methods. Microchemical Journal, v. 98, n. 1, p. 135-143, MAY 2011. (05/00294-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

GALLI, Andressa. Development and characterization of the byenzimatic biosensor immobilized on self assembled monolayers to determination of the sugars in food. 2009. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Química de São Carlos (IQSC/BT) São Carlos.