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Development and characterization of a bienzymatic biosensor to determine sugars in dietetic food

Grant number: 05/00294-8
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): October 01, 2005
Effective date (End): February 28, 2009
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
Principal researcher:Sergio Antonio Spinola Machado
Grantee:Andressa Galli
Home Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil


This project aim to develop, characterize and apply a bienzimatic biosensors prepared by modifying a polycrystalline Au electrode with a self-assembly monolayer of mercaptopropionic acid and mercaptoundecanoic acid. This monolayer will be further modified by glucose oxidase, fructose dehydrogenase enzymes and tetrathiafulvalene adsorption, for the electrochemical determination of sugars (glucose and fructose) in dietetic foods. The determinations using the enzymes modified electrodes will be made by chronoamperometric method, using the electrochemical oxidation of an espeficic mediator (tetrathiafulvalene for glucose oxidase and 3,4-dihydroxibenzaldehide for the fructose dehydrogenase enzymes, respectively) whose oxidation state is changed by the individual action of the enzymatic in its specific substrate. The electrochemical determinations (by square wave voltammetric) of the mediators will yield an electroanalytical method to the used for sugar determination in dietetic foods. The influence of some possible contaminants in these matrices will be also analyzed. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GALLI, ANDRESSA; DE SOUZA, DJENAINE; MACHADO, SERGIO A. S. Pendimethalin determination in natural water, baby food and river sediment samples using electroanalytical methods. Microchemical Journal, v. 98, n. 1, p. 135-143, MAY 2011. Web of Science Citations: 16.
DJENAINE DE SOUZA; ANDRESSA GALLI; MARCELO L. CALEGARO; SERGIO A. S. MACHADO; ROBERTO C. PIRES. Utilização da múltipla voltametria de onda quadrada na determinação eletroanalítica de compostos orgânicos e inorgânicos. Química Nova, v. 30, n. 2, p. 458-463, Abr. 2007.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
GALLI, Andressa. Desenvolvimento e caracterização de um biossensor bienzimático imobilizado sobre monocamadas auto-organizadas para determinação de açúcares em alimentos. 2009. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Química de São Carlos São Carlos.

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