Evaluation of immunotoxic potential of diuron herbicide: acute and sub-chronic toxicity study of repeated doses

• Grant number: 05/01223-7
• Support Opportunities: Scholarships in Brazil - Master
• Start date: September 01, 2005
• End date: February 28, 2007
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Luís Fernando Barbisan
• Grantee: Alexandre Domingues
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Diuron, N-(3,4-dichlorophenyl)-N,N-dimethyl urea, is a phenylamide herbicide, belonging to the subclass of phenylurea. In Brazil, this substitute of urea herbicides has been used on many agricultural crops such as cofee, fruits, cotton, sugar cane, alfafa and wheat. Diuron is considered a Priority Hazardous Substance by the European Commision, being classified as II – IV toxicological level.In carcinogenicity evaluations this herbicide increases the incidence of some types of tumors in NMR1 mices anda Wistar rats. Many studies has been showed that herbicides affect the immunological system, the interaction of xenobiotics and immune components is able to take to a immunological dysfunction in the host and, for consequence, increased chances of auto-immunes diseases and hipersensibility reactions or even neoplasias.The objective of this studie is to evaluate the immunotoxical potencial of Diuron in Wistar rats, observing general parameters of toxity ( body and organ weights , water and ration consumption ) , biochemistries analyse of creatinin and alanin aminotrasferasis (ALT), hematological parameters ( global and relative count of blood and bone marrow cells), linfohematopoetics, liver and kidneys organs histology and immunological parameters ( linfocites phenotype, citokine dosages and linfoproliferative aspect). The studie has 4 major groups of Wistar rats: 1- Negative control, 2- Positive Control, 3,4,5- Diuron treated group (different doses) and 6- Diuron treated group until 90 days and then a stop period ( in administration) before sacrifictaion. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DOMINGUES, ALEXANDRE; BARBISAN, LUIS FERNANDO; MARTINS, PRISCILA RAQUEL; TOZZI SPINARDI-BARBISAN, ANA LUCIA. Diuron exposure induces systemic and organ-specific toxicity following acute and sub-chronic exposure in male Wistar rats. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, v. 31, n. 3, p. 387-396, MAY 2011. (05/01223-7)

DOMINGUES, ALEXANDRE; GRASSI, TONY F.; SPINARDI-BARBISAN, ANA L. T.; BARBISAN, LUIS F.. Developmental exposure to diuron causes splenotoxicity in male Sprague-Dawley rat pups. JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMIN, v. 47, n. 5, p. 420-426, 2012. (06/01330-0, 05/01223-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

DOMINGUES, Alexandre. Avaliação do potencial imunotóxico do herbicida diuron: estudo de toxicidades de 28 e 90 dias(doses repetidas). 2007. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina. Botucatu Botucatu.