Effect of periodontal microbiota on differentiation and function of dendritic cells from patients with aggressive periodontitis

• Grant number: 07/02030-3
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2008
• End date: May 31, 2009
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Patricia Ramos Cury
• Grantee: Ana Elisa Amaro Rodrigues
• Host Institution: Centro de Pesquisas Odontológicas São Leopoldo Mandic. Faculdade São Leopoldo Mandic (SLMANDIC). Sociedade Regional de Ensino e Saúde S/S Ltda (SRES). Campinas , SP, Brazil

Abstract

The aim of this study is to evaluate the effect of red complex bacterial species of periodontal microbiota and Aggregatibacter actinomycetemcomitans on the membrane phenotype and function of dendritic cells (DCs) maturated in vitro from peripheral blood mononuclear cells (PBMC) of the patients with aggressive periodontitis. Cultured human monocyte-derived dendritic cells (MDDCs) from periodontally-healthy and aggressive periodontitis subjects will be pulsed with extract of Tannarella forsythia, Porphyromonas gingivalis, Treponema denticola e Aggregatibacter actinomycetemcomitans. The effect of this treatment under the immunostimulate capacity of DCs will be evaluated in co-cultures with allogeneic T cells. The following cytokines will be quantified on the supernatant through ELISA test: IFN-γ, IL-10, TNF, IL-1 and IL-12. To determinate the cells’ phenotype, they will be marked with monoclonais antibodies: anti-CD1a, anti-CD14, anti-CD80, anti-CD86, anti-CD83, anti-CD11c, anti-CCR7 and class II- anti-HLA.