Paecilomyces variotii tanase immobilization and quimioselectivity studies for hydrolisys and sinthesys reactions

• Grant number: 09/07576-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: October 01, 2009
• End date: January 31, 2011
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Science
• Principal Investigator: Gabriela Alves Macedo
• Grantee: Patrícia Fernanda Schons
• Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Tannases (E.C. 3.1.1.20) are esterases who hydrolyse the "ester" bond and the "depside" bond in substrates such as tannic acid, epicatechin gallate, epigallocatechin gallate in aqueous medium and synthesize esters of gallic acid in medium containing alcool and organic solvent. Enzymes are used in various industrial sectors, however, their use often is limited due to its low stability and high cost, one way to solve this problem is through the enzyme immobilization. The synthesis of gallates is important for producing natural compounds with high antioxidant capacity, which can be used in place of those as synthetic BHA, BHT and TBHQ. This project aims to study and optimize the immobilization of the enzyme tannase by Paecilomyces variotti in different suports. Than free and immobilized enzyme will be used to study the synthesis of esters of gallic acid and the hydrolisis of pure (commercial) galic and elagic phenolic compounds . The reactions of synthesis of esters will be optimized using experimental design, the variables under study for the synthesis will be concentration of substrate and enzyme, temperature, agitation, pH, concentration of water and sorbitol and for hydrolysis concentration of substrate and enzyme, temperature, agitation and pH. The reaction will be monitored after 2, 4, 6, 12 and 24 hours of the start of the experiment.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SCHONS, Patrícia Fernanda. Immobilization of tannase from Pecilomyces variotii and reactions of hydrolysis and synthesis. 2012. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia de Alimentos Campinas, SP.