Association between red fluorescence from dental biofilm and caries activity

• Grant number: 09/16082-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2010
• End date: May 31, 2013
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Fausto Medeiros Mendes
• Grantee: Tatiane Fernandes de Novaes
• Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

This study aims: (1) to evaluate in vivo the reproducibility and validity of QLF and DIAGNOdent pen in detecting caries lesions activity on occlusal surface of primary molars and to test the association between caries activity and the presence of red fluorescent bacterial plaque; (2) to evaluate in vivo the validity of QLF and DIAGNOdent pen in relation to caries activity in primary molars; (3) to evaluate the influence of red fluorescent biofilm as predictor of caries in erupting first permanent molars. For the aim (1), one hundred fifty children between 3 and 9 years old, presenting at least one primary molar, will be selected. They will be examined for the presence of plaque and bleeding, caries experience and evaluation of red fluorescent biofilm with the QLF. In a second session, the teeth will be reevaluated in relation to caries activity using the ICDAS II, QLF and DIAGNOdent pen. The validation of these methods will be made by consensus of the investigators using the ICDAS II. For the aim (2), from sample of first objective, 50 extracted or exfoliated teeth will be sectioned and histologically validated using pH indicator dye. Finally, for the aim (3), one hundred children presenting at least one first permanent molar eruption without caries will be selected. The same clinical parameters of the protocol I will be evaluated. After one week, the QLF and DIAGNOdent pen will be employed for evaluation of red fluorescent biofilm and the loss of fluorescence observed. The children will be followed-up at 1, 3, 6, 9 and 12 months after the initial clinical examination.

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NOVAES, TATIANE F.; MATOS, RONILZA; GIMENEZ, THAIS; BRAGA, MARIANA M.; DE BENEDETTO, MONIQUE S.; MENDES, FAUSTO M.. Performance of fluorescence-based and conventional methods of occlusal caries detection in primary molars - an in vitro study. International Journal of Paediatric Dentistry, v. 22, n. 6, p. 459-466, NOV 2012. (09/16082-0)

TATIANE FERNANDES NOVAES; RONILZA MATOS; PAULA CELIBERTI; MARIANA MINATEL BRAGA; FAUSTO MEDEIROS MENDES. The influence of interdental spacing on the detection of proximal caries lesions in primary teeth. Brazilian Oral Research, v. 26, n. 4, p. 293-299, 2012-08-00. (09/16082-0)

NOVAES, TATIANE FERNANDES; MATOS, RONILZA; CELIBERTI, PAULA; BRAGA, MARIANA MINATEL; MENDES, FAUSTO MEDEIROS. The influence of interdental spacing on the detection of proximal caries lesions in primary teeth. BRAZILIAN ORAL RESEARCH, v. 26, n. 4, p. 7-pg., 2012-07-01. (09/16082-0)

NOVAES, TATIANE FERNANDES; MATOS, RONILZA; CELIBERTI, PAULA; BRAGA, MARIANA MINATEL; MENDES, FAUSTO MEDEIROS. The influence of interdental spacing on the detection of proximal caries lesions in primary teeth. Brazilian Oral Research, v. 26, n. 4, p. 293-299, JUL-AUG 2012. (09/16082-0)

BITTAR, DANIELA G.; PONTES, LAURA REGINA A.; CALVO, ANA FLAVIA B.; NOVAES, TATIANE F.; BRAGA, MARIANA M.; FREITAS, PATRICIA M.; TABCHOURY, CINTHIA P. M.; MENDES, FAUSTO M.. Is the red fluorescence of dental plaque related to its cariogenicity?. Journal of Biomedical Optics, v. 19, n. 6, JUN 2014. (09/16082-0)

NOVAES, TATIANE FERNANDES; MATOS, RONILZA; RAGGIO, DANIELA PROCIDA; BRAGA, MARIANA MINATEL; MENDES, FAUSTO MEDEIROS. Children's discomfort in assessments using different methods for approximal caries detection. Brazilian Oral Research, v. 26, n. 2, p. 93-99, MAR-APR 2012. (09/16082-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

NOVAES, Tatiane Fernandes de. Use of Fluorescence-based methods to assess the caries activity on occlusal surfaces of primary molars. 2013. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Odontologia (FO/SDO) São Paulo.