Purification and characterization of compounds isolated from plants with larvicidal activity.

• Grant number: 09/17058-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: May 01, 2010
• End date: April 30, 2014
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal Investigator: Maria Luiza Vilela Oliva
• Grantee: Natalia Neto dos Santos Nunes
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Aedes aegypti transmits etiologic agents of yellow fever and dengue. Vaccine for dengue virus is not available and vector control is essential to minimize dengue incidence. Aqueous extracts 1% (p/v) of the seed tegument and cotyledon of Carica did not show larvicidal activity when tested separately. However, the mixture of both caused 100% of larval mortality at concentration of approximately 5mg/mL, in bioassay. This project aims the characterization and the establishment of the mechanism of action of the toxic activity found in Carica seeds on the larvae of A. aegypti.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NUNES, NATALIA N. DOS S.; SANTANA, LUCIMEIRE A.; SAMPAIO, MISAKO U.; LEMOS, FRANCISCO J. A.; OLIVA, MARIA LUIZA. The component of Carica papaya seed toxic to A-aegypti and the identification of tegupain, the enzyme that generates it. Chemosphere, v. 92, n. 4, p. 413-420, JUL 2013. (09/17058-6, 09/53766-5)

NUNES, NATALIA N. S.; FERREIRA, RODRIGO S.; SILVA-LUCCA, ROSEMEIRE A.; DE SA, LEONARDO F. R.; DE OLIVEIRA, ANTONIA ELENIR A.; CORREIA, MARIA TEREZA DOS S.; PAIVA, PATRICIA MARIA G.; WLODAWER, ALEXANDER; OLIVA, MARIA LUIZA V.. Potential of the Lectin/Inhibitor Isolated from Crataeva tapia Bark (CrataBL) for Controlling Callosobruchus maculatus Larva Development. Journal of Agricultural and Food Chemistry, v. 63, n. 48, p. 10431-10436, DEC 9 2015. (09/17058-6, 09/53766-5)