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Interaction and toxicity effects of Vip3Aa and Cry1Ac proteins from Bacillus thuringiensis on populations of Diatraea saccharalis (Fabr., 1794) (LEPIDOPTERA: PYRALIDAE)

Grant number: 09/18544-1
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2010
Effective date (End): January 31, 2014
Field of knowledge:Agronomical Sciences - Agronomy - Plant Health
Principal Investigator:Manoel Victor Franco Lemos
Grantee:Camila Chiaradia Davolos
Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil


Bacillus thuringiensis insecticidal proteins are widely used as biopesticides. However, the resistance evolution on insect pests is the biggest obstacle to the successful application of these insecticides on biological control programs. To avoid this process is necessary to understand how the mode of action of these toxins is. Studies evolving different toxin proteins of B. thuringiensis and their interactions with membrane receptors of the intestinal epithelium at target insects are very important to evaluate strategies that may prevent or control the resistance evolution of these pests to toxins. Therefore, the aim of this research is to verify the toxicity of Cry and VIP proteins of B. thuringiensis (crystal and vegetative insecticidal proteins) on two populations of Diatraea saccharalis, prioring the toxicity and biochemical analysis of the mode of action of these toxins, in order to prevent the resistance evolution on insect pests.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DAVOLOS, CAMILA C.; HERNANDEZ-MARTINEZ, PATRICIA; CRIALESI-LEGORI, PAULA C. B.; DESIDERIO, JANETE A.; FERRE, JUAN; ESCRICHE, BALTASAR; LEMOS, MANOEL VICTOR F.. Binding analysis of Bacillus thuringiensis Cry1 proteins in the sugarcane borer, Diatraea saccharalis (Lepidoptera: Crambidae). Journal of Invertebrate Pathology, v. 127, p. 32-34, . (09/18544-1)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
DAVOLOS, Camila Chiaradia. Efeitos da interação e toxicidade das proteínas Cry1 e Vip3A de Bacillus thuringiensis em Diatraea saccharalis (Fabr., 1794) (Lepidoptera: Crambidae). 2014. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.

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