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Virulence genotypes, antimicrobial susceptibility profiles, and molecular typing of Staphylococcus aureus associated to mastitis in animals coming from the States of Paraná and São Paulo

Grant number: 10/02038-7
Support type:Scholarships in Brazil - Master
Effective date (Start): August 01, 2010
Effective date (End): February 29, 2012
Field of knowledge:Agronomical Sciences - Animal Husbandry - Animal Production
Principal researcher:Juliana Rodrigues Pozzi Arcaro
Grantee:Lívia Castelani
Home Institution: Instituto de Zootecnia. Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Nova Odessa , SP, Brazil

Abstract

The purpose of this study is to investigate virulence genotypes, antimicrobial susceptibility profiles, and molecular typing of Staphylococcus aureus isolates associated to mastitis in heifers, after it and lactation. The study will be performed on calves acquired from the state of Paraná - their ages ranging from 15 days to 3 months - which, after being inseminated, around 15 months, they are transferred to milk producers who are part of the project "Balde Cheio". Also, with calves once again acquired in the state of Paraná, from the "Centro Apta Bovinos de Leite", and the "Instituto de Zootecnia", located in Nova Odessa. The milk samples will be taken from black and white Holstein calves and cows in lactation, 60 days before calving, in the day of the birth, 10 days after the calving and during lactation. The samples will be extracted for phenotypic and genotypic identification of Staphylococcus aureus. Antimicrobial susceptibility profiles will be determined by using a disk diffusion method and virulence genes will be screened by PCR. Finally, clonal relationship will be investigated by pulsed field gel electrophoresis (PFGE). The statistical analysis of the microbiological results will be done using the (c²) test for comparison of percentage values (qualitative variables) when n is higher than 5 and the Fischer´s exact test when n is 5 or less. A statistical significance will be defined by a p value d 0.05. The evaluation of discriminatory capacity will be determined according to the numerical index described by Hunter & Gaston (1988). (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CASTELANI, LIVIA; PILON, LUCAS EDUARDO; MARTINS, THAMIRES; POZZI, CLAUDIA RODRIGUES; POZZI ARCARO, JULIANA RODRIGUES. Investigation of biofilm production and icaA and icaD genes in Staphylococcus aureus isolated from heifers and cows with mastitis. ANIMAL SCIENCE JOURNAL, v. 86, n. 3, p. 340-344, . (10/02038-7)
CASTELANI, LIVIA; SILVA SANTOS, ALINE FRANCIELE; MIRANDA, MARIANA DOS SANTOS; ZAFALON, LUIZ FRANCISCO; POZZI, CLAUDIA RODRIGUES; POZZI ARCARO, JULIANA RODRIGUES. Molecular Typing of Mastitis-Causing Staphylococcus aureus Isolated from Heifers and Cows. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 14, n. 2, p. 4326-4333, . (10/02038-7)

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