Morphology, anatomy and phytochemistry of species of subtribe Lychnophorinae (Asteraceae: Vernonieae) as subsidies for the phylogenetic analysis of group

• Grant number: 10/02085-5
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: May 01, 2010
• End date: August 31, 2014
• Field of knowledge: Biological Sciences - Botany - Plant Morphology
• Principal Investigator: Beatriz Appezzato da Glória
• Grantee: Makeli Garibotti Lusa
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated scholarship(s): 12/16071-1 - Morphoanatomic adaptations of species of Lychnophorinae subtribe (Asteraceae: Vernonieae) to environmental conditions, BE.EP.DR

Abstract

Asteraceae is the largest family among angiosperms, with about 1,600genera and 23,000 species. The family has 20 tribes, and Vernonieae groupthe least understood. Within Lychnophorinae there are several taxa notinvestigated chemically, as well as uncertainty in the phylogeneticposition of the same. The sesquiterpene lactones are substances that maybe related to protection against herbivory. Due to their structuralvariety, many of them are considered chemotaxonomic markers of members ofAsteraceae, including subtribes and genera of Lychnophorinae, whichcertainly can serve as a valuable tool to aid in phylogeny. For thisstudy, were elected key species within Lychnophorinae. The secondarymetabolites are generally associated with secretory structures. InAsteraceae, the taxonomic value of these structures is undeniable.Structural analysis of the elected species may provide importantindications on the evolution of structure in the group. Thus, theobjectives of this study are: to provide new data for phylogeneticanalysis of Lychnophorinae already underway, to identify the site ofsecretion of secondary metabolites, and understand the development ofsecretory structures and the chemical evolution of the group. Thecollections will be held in 'Serra da Canastra', 'Serra do Cipó' and'Serra do Caraça' at Minas Gerais State, and in 'Morro do Chapéu' and'Chapada Diamantina' at the State of Bahia, Brazil. For the anatomicalanalysis, samples of stem and leaves will be collected from adult plants.Phytochemical analysis will be performed using high performance liquidchromatography (HPLC) using a monolithic column for reversed phase. Thedata obtained from these tests will be compared with the availableliterature.

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LUSA, MAKELI GARIBOTTI; PATRICE LOEUILLE, BENOIT FRANCIS; APPEZZATO-DA-GLORIA, BEATRIZ. First record of phytomelanin in aerial vegetative organs and its evolutionary implications in Lychnophorinae (Vernonieae: Asteraceae). PERSPECTIVES IN PLANT ECOLOGY EVOLUTION AND SYSTEMATICS, v. 33, p. 18-33, AUG 2018. (10/51454-3, 10/02085-5)

LUSA, MAKELI G.; DA COSTA, FERNANDO B.; APPEZZATO-DA-GLORIA, BEATRIZ. Histolocalization of chemotaxonomic markers in Brazilian Vernonieae (Asteraceae). Botanical Journal of the Linnean Society, v. 182, n. 3, p. 581-593, NOV 2016. (10/51454-3, 10/02085-5)

LUSA, MAKELI G.; MARTUCCI, MARIA E. P.; LOEUILLE, BENOIT F. P.; GOBBO-NETO, LEONARDO; APPEZZATO-DA-GLORIA, BEATRIZ; DA COSTA, FERNANDO B.. Characterization and evolution of secondary metabolites in Brazilian Vernonieae (Asteraceae) assessed by LC-MS fingerprinting. Botanical Journal of the Linnean Society, v. 182, n. 3, p. 594-611, NOV 2016. (10/51454-3, 10/02085-5)

LUSA, MAKELI GARIBOTTI; APPEZZATO-DA-GLORIA, BEATRIZ; LOEUILLE, BENOIT; BARTOLI, GIACOMO; CICCARELLI, DANIELA. Functional groups in Lychnophorinae (Asteraceae: Vernonieae) based on morphological and anatomical traits. Australian Journal of Botany, v. 62, n. 2, p. 150-163, 2014. (12/16071-1, 10/51454-3, 10/02085-5)

LUSA, MAKELI GARIBOTTI; CARDOSO, ELAINE CRISTINA; MACHADO, SILVIA RODRIGUES; APPEZZATO-DA-GLORIA, BEATRIZ. Trichomes related to an unusual method of water retention and protection of the stem apex in an arid zone perennial species. AOB PLANTS, v. 7, 2015. (10/51454-3, 10/02085-5)

LUSA, MAKELI GARIBOTTI; PATRICE LOEUILLE, BENOIT FRANCIS; CICCARELLI, DANIELA; APPEZZATO-DA-GLORIA, BEATRIZ. Evolution of Stem and Leaf Structural Diversity: a Case Study in Lychnophorinae (Asteraceae). BOTANICAL REVIEW, v. 84, n. 3, p. 203-241, SEP 2018. (10/51454-3, 10/02085-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LUSA, Makeli Garibotti. Morphoanatomy and phytochemistry of species of Lychnophorinae subtribe (Asteraceae: Vernonieae) as subsidies for the phylogenetic analyses of group. 2014. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.