Asteraceae is the largest family among angiosperms, with about 1,600genera and 23,000 species. The family has 20 tribes, and Vernonieae groupthe least understood. Within Lychnophorinae there are several taxa notinvestigated chemically, as well as uncertainty in the phylogeneticposition of the same. The sesquiterpene lactones are substances that maybe related to protection against herbivory. Due to their structuralvariety, many of them are considered chemotaxonomic markers of members ofAsteraceae, including subtribes and genera of Lychnophorinae, whichcertainly can serve as a valuable tool to aid in phylogeny. For thisstudy, were elected key species within Lychnophorinae. The secondarymetabolites are generally associated with secretory structures. InAsteraceae, the taxonomic value of these structures is undeniable.Structural analysis of the elected species may provide importantindications on the evolution of structure in the group. Thus, theobjectives of this study are: to provide new data for phylogeneticanalysis of Lychnophorinae already underway, to identify the site ofsecretion of secondary metabolites, and understand the development ofsecretory structures and the chemical evolution of the group. Thecollections will be held in 'Serra da Canastra', 'Serra do Cipó' and'Serra do Caraça' at Minas Gerais State, and in 'Morro do Chapéu' and'Chapada Diamantina' at the State of Bahia, Brazil. For the anatomicalanalysis, samples of stem and leaves will be collected from adult plants.Phytochemical analysis will be performed using high performance liquidchromatography (HPLC) using a monolithic column for reversed phase. Thedata obtained from these tests will be compared with the availableliterature.
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