Cloning and expression of human protein kinase Nek10 structural and functional studies aimed at molecular and cellular

• Grant number: 10/15262-2
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: February 01, 2011
• End date: July 31, 2015
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Jörg Kobarg
• Grantee: Priscila Ferreira Papa
• Host Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia e Inovação (Brasil). Campinas , SP, Brazil
• Associated scholarship(s): 13/11160-9 - hNek10 protein kinase functional and interaction profile in DNA damage repair, BE.EP.DD

Abstract

The family of protein kinases NIMA (never in mitosis, gene A) was discovered in Aspergillus nidulans as a serine/threonine kinase highly conserved and important for the functioning of the cell cycle. The family of Neks (NIMA related kinases) found in mammals shows 40% identity with the kinase domain of these proteins. Thus, it plays a similar role in cell cycle progression, in general, at regions of checkpoints during interphase providing its functional relationship with motility of breast and colorectal cancer, polycystic kidney disease, among other pathologies. Protein Nek10 has not yet solved its structural features in literature, however, recently the gene loci of Nek10 was identified as susceptible to the cause of breast cancer, highlighting the importance of his study for possible treatment of this disease. For this purpose, the protein Nek10 will be cloned, expressed and purified using techniques of molecular biology to lead experiments involving the interaction, when it is phosphorylated, with other proteins and signaling pathways, and silencing its investigation of potential therapeutic targets related Nek10. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MELO-HANCHUK, TALITA DINIZ; SLEPICKA, PRISCILA FERREIRA; PELEGRINI, ALESSANDRA LUIZA; MARTINS MENCK, CARLOS FREDERICO; KOBARG, JORG. NEK5 interacts with topoisomerase II beta and is involved in the DNA damage response induced by etoposide. Journal of Cellular Biochemistry, v. 120, n. 10, p. 16853-16866, OCT 2019. (14/15982-6, 17/03489-1, 10/51730-0, 15/06458-4, 10/16831-0, 10/15262-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PAPA, Priscila Ferreira. Characterization of the hNek5 and hNek10 proteins in the DNA damage response context: a functional and interatomic approach. 2015. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.