Influence of periodontal pathogens on antimicrobial peptide LL37 expression in gingival tissue and neutrophils from healthy and periodontally diseased individuals

• Grant number: 07/00219-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: November 01, 2007
• End date: April 30, 2010
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Reginaldo Bruno Gonçalves
• Grantee: Flávia Sammartino Mariano Rodrigues
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

Human antimicrobial peptides as LL37 presents a broad spectrum of bactericidal activity against to Gram-positive and Gram-negative bacteria, fungi and enveloped viruses, thus, represent an important defense mechanism against the pathogenic microorganisms invasion. They appear to be engaged in the regulation of both innate and adaptive immune functions in oral cavity. Our aims are to evaluate of LL37 levels in the gingival tissue and in the neutrophils culture isolated from peripheral blood from volunteers with non-inflamed and inflamed gingival. The levels of LL37cathelicidin in gingival tissue will be evaluated by ELISA method using a synthetic peptide and specific policlonal antibodies. Additionally, to study the bacterial interference with the LL37expression and production by neutrophils, these cells will be stimulated with a several bacteria and by some virulence factors. The supernatant of cultures will be submitted to ELISA method to LL37quantification and the cellular mRNA extracted will be used to quantification of cathelicidin through the reaction chain of polymerize in real time (Real Time-PCR) technique.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARIANO, F. S.; CAMPANELLI, A. P.; NOCITI, JR., F. H.; MATTOS-GRANER, R. O.; GONCALVES, R. B.. Antimicrobial peptides and nitric oxide production by neutrophils from periodontitis subjects. Brazilian Journal of Medical and Biological Research, v. 45, n. 11, p. 1017-1024, NOV 2012. (07/00219-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

RODRIGUES, Flávia Sammartino Mariano. Periodontopathogens influence in the expression and production of antimicrobial peptides and inflammatory cytokines in neutrophils from healthy and periodontitis individuals. 2010. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba Piracicaba, SP.