On the chemichal and physical endodontic methods to decontaminate intraorally infected dentin

• Grant number: 10/16002-4
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: February 01, 2011
• End date: June 30, 2013
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Clovis Monteiro Bramante
• Grantee: Ronald Ordinola Zapata
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Associated scholarship(s): 11/22283-9 - Efficacy of photon initiated acoustic streaming to kill biofilms attached to dentin, BE.EP.DR

Abstract

The aim of this study is to evaluate the effect of sodium hypochlorite on the structural characteristics and viability of microbial cells in biofilms formed on dentin according to the concentration of sodium hypochlorite (1% vs 2.5%), exposure time (5, 15 and 30 minutes), use of chelating solutions, intracanal dressings, root canal dressings, ultrasound or lasers. Sterile bovine dentin blocks will be mounted on a palatal intraoral appliance, which will be used by a volunteer for a period from 72 to 96 hours for the formation of oral biofilm in situ. Then, the dentin blocks will be carefully removed from and washed with 100 mL of PBS. The blocks will be experimentally treated with sodium hypochlorite using different concentrations and times. After the experiment, 500¼l of sodium thiosulfate was used to eliminate waste and inactivate the sodium hypochlorite solution. The samples will be stained with 50 ul of a solution of propidium iodide/Syto9, Live / Dead technique (marker of cell membrane integrity) for 10 minutes for the subsequent analysis of the vitality by using a Confocal Laser Scanning microscope.For the experiments that include the use of ultrasound or lasers will be used transparent acrylic teeth with artificial canal. An external cavity which communicates with the artificial root canal will position the block of infected dentin to simulate the conditions after the intracanal use of ultrasound.Statistical analysis of the values obtained for each group will be calculated by using the "GraphPad Prism" software.

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VILLAS-BOAS, MARCELO HAAS; BERNARDINELI, NORBERTI; CAVENAGO, BRUNO CAVALINI; MARCIANO, MARINA; DEL CARPIO-PEROCHENA, ALDO; DE MORAES, IVALDO GOMES; DUARTE, MARCO H.; BRAMANTE, CLOVIS M.; ORDINOLA-ZAPATA, RONALD. Micro-Computed Tomography Study of the Internal Anatomy of Mesial Root Canals of Mandibular Molars. JOURNAL OF ENDODONTICS, v. 37, n. 12, p. 1682-1686, DEC 2011. (10/16002-4)

ORDINOLA-ZAPATA, RONALD; BRAMANTE, CLOVIS MONTEIRO; VILLAS-BOAS, MARCELO HAAS; CAVENAGO, BRUNO CAVALINI; DUARTE, MARCO HUNGARO; VERSIANI, MARCO AURELIO. Morphologic Micro-Computed Tomography Analysis of Mandibular Premolars with Three Root Canals. JOURNAL OF ENDODONTICS, v. 39, n. 9, p. 1130-1135, SEP 2013. (09/09989-0, 10/16072-2, 10/16002-4)

DEL CARPIO-PEROCHENA, ALDO E.; BRAMANTE, CLOVIS M.; DUARTE, MARCO A. H.; CAVENAGO, BRUNO CAVALINI; VILLAS-BOAS, MARCELO H.; GRAEFF, MARCIA S.; BERNARDINELI, NORBERTI; DE ANDRADE, FLAVIANA BOMBARDA; ORDINOLA-ZAPATA, RONALD. Biofilm Dissolution and Cleaning Ability of Different Irrigant Solutions on Intraorally Infected Dentin. JOURNAL OF ENDODONTICS, v. 37, n. 8, p. 1134-1138, AUG 2011. (09/03217-5, 10/16002-4)

ORDINOLA-ZAPATA, R.; BRAMANTE, C. M.; APRECIO, R. M.; HANDYSIDES, R.; JARAMILLO, D. E.. Biofilm removal by 6% sodium hypochlorite activated by different irrigation techniques. International Endodontic Journal, v. 47, n. 7, p. 659-666, JUL 2014. (11/22283-9, 10/16002-4)

ORDINOLA-ZAPATA, RONALD; BRAMANTE, CLOVIS M.; GARCIA, ROBERTO BRANDAO; DE ANDRADE, FLAVIANA BOMBARDA; BERNARDINELI, NORBERTI; DE MORAES, IVALDO GOMES; DUARTE, MARCO A. H.. The antimicrobial effect of new and conventional endodontic irrigants on intra-orally infected dentin. ACTA ODONTOLOGICA SCANDINAVICA, v. 71, n. 3-4, p. 424-431, MAY-JUL 2013. (10/16002-4)

ORDINOLA-ZAPATA, RONALD; BRAMANTE, CLOVIS M.; MINOTTI, PALOMA GAGLIARDI; CAVENAGO, BRUNO CAVALINI; GARCIA, ROBERTO BRANDAO; BERNARDINELI, NORBERTI; JARAMILLO, DAVID E.; DUARTE, MARCO A. HUNGARO. Antimicrobial Activity of Triantibiotic Paste, 2% Chlorhexidine Gel, and Calcium Hydroxide on an Intraoral-infected Dentin Biofilm Model. JOURNAL OF ENDODONTICS, v. 39, n. 1, p. 115-118, JAN 2013. (10/16002-4)

ORDINOLA-ZAPATA, RONALD; BRAMANTE, CLOVIS MONTEIRO; HUNGARO DUARTE, MARCO ANTONIO; CAVENAGO, BRUNO CAVALINI; JARAMILLO, DAVID; VERSIANI, MARCO AURELIO. Shaping ability of Reciproc and TF Adaptive systems in severely curved canals of rapid microCT-based prototyping molar replicas. Journal of Applied Oral Science, v. 22, n. 6, p. 509-515, NOV-DEC 2014. (13/03695-0, 10/16072-2, 09/09989-0, 10/16002-4)

CAVENAGO, B. C.; ORDINOLA-ZAPATA, R.; DUARTE, M. A. H.; DEL CARPIO-PEROCHENA, A. E.; VILLAS-BOAS, M. H.; MARCIANO, M. A.; BRAMANTE, C. M.; MORAES, I. G.. Efficacy of xylene and passive ultrasonic irrigation on remaining root filling material during retreatment of anatomically complex teeth. International Endodontic Journal, v. 47, n. 11, p. 1078-1083, NOV 2014. (11/18272-1, 10/16072-2, 10/16002-4)

ORDINOLA-ZAPATA, R.; BRAMANTE, C. M.; CAVENAGO, B.; GRAEFF, M. S. Z.; GOMES DE MORAES, I.; MARCIANO, M.; DUARTE, M. A. H.. Antimicrobial effect of endodontic solutions used as final irrigants on a dentine biofilm model. International Endodontic Journal, v. 45, n. 2, p. 162-168, FEB 2012. (10/16002-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ZAPATA, Ronald Ordinola. Efeito da irrigação e medicação endodôntica em dentina infectada por biofilmes orais. 2013. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB) Bauru.