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Microbiological evaluation of periodontal and implant-related sites in titanium and zirconia abutments using the DNA Checkerboard method and 16S rDNA-based PCR

Grant number: 10/10442-2
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): January 01, 2011
Effective date (End): December 31, 2013
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal researcher:Ricardo Faria Ribeiro
Grantee:Cássio Do Nascimento
Home Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


Molecular diagnostic methods using genetic material have been largely employed to detect and quantify species harbouring the oral cavity. The aim of this study will be to evaluate the microbial composition of subgingival biofilm from teeth and implants sulci in relation to contents originated from internal parts of the implant and abutments fabricated in titanium or zirconia. Twenty partially edentulous healthy patients will be enrolled in this study. Bacterial counts and percentage of colonized sites will be evaluated using the DNA checkerboard method and 16S rDNA-based PCR. Pearson's correlation tests will be performed to investigate the correlation between microbial counts from the subgingival dental biofilm and the biofilm from implant, abutments, and implant prosthesis.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DO NASCIMENTO, CASSIO; PITA, MURILLO SUCENA; SANTOS, EMERSON DE SOUZA; MONESI, NADIA; PEDRAZZI, VINICIUS; DE ALBUQUERQUE JUNIOR, RUBENS FERREIRA; RIBEIRO, RICARDO FARIA. Microbiome of titanium and zirconia dental implants abutments. Dental Materials, v. 32, n. 1, p. 93-101, JAN 2016. Web of Science Citations: 20.
DO NASCIMENTO, CASSIO; PITA, MURILLO SUCENA; NOGUEIRA, FLAVIO HENRIQUE; FERNANDES, CARRICO; PEDRAZZI, VINICIUS; DE ALBUQUERQUE JUNIOR, RUBENS FERREIRA; RIBEIRO, RICARDO FARIA. Bacterial adhesion on the titanium and zirconia abutment surfaces. Clinical Oral Implants Research, v. 25, n. 3, p. 337-343, MAR 2014. Web of Science Citations: 56.
DO NASCIVNENTO, CASSIO; PITA, MURILLO SUCENA; PEDRAZZI, VINICIUS; DE ALBUQUERQUE JUNIOR, RUBENS FERREIRA; RIBEIRO, RICARDO FARIA. In vivo evaluation of Candida spp. adhesion on titanium or zirconia abutment surfaces. ARCHIVES OF ORAL BIOLOGY, v. 58, n. 7, p. 853-861, JUL 2013. Web of Science Citations: 9.

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