|Support type:||Scholarships in Brazil - Doctorate|
|Effective date (Start):||May 01, 2011|
|Effective date (End):||April 30, 2015|
|Field of knowledge:||Physical Sciences and Mathematics - Chemistry - Organic Chemistry|
|Principal Investigator:||Ian Castro-Gamboa|
|Home Institution:||Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil|
AbstractThe search for new sources of natural products have been responsible for the increased use of analytical tools that enable a rapid and efficient analysis of these complex matrixes and is of fundamental importance in the current bioprospecting programs. In this context, the methodologies associated with dereplication methodologies coupled to spectroscopic state of the art technology as well as different in vitro and in vivo bioassays are accelerating the choosing of biologically promising extracts. Our research group "NuBBE" (Nuclei of Bioassays, Biosynthesis and Ecophysiology of Natural Products) has incorporated experimental strategies and chromatographic methods to select, from our bank of extract, lead candidates for further studies. Recently the use of high performance liquid chromatography (HPLC) hyphenated with high resolution mass spectrometry (HRMS) allowed us to analyze plant and endophytic fungi extracts speeding up the selection of biologically active fractions as well as assisting on chemical composition studies of the known bioactive chemotypes and selecting those with novel molecular characteristics for further separation and purification. Innovative spectroscopic techniques using Nuclear Magnetic Resonance (NMR), recently applied on the study of dynamic complex matrixes, are being integrated with HPLC methods mainly due to their robustness, low sample amounts and spectroscopic versatilities. This project aims to understand the molecular relationships present in complex matrices generating a rational approach to study the relationship between the rhizosphere and microorganisms that inhabit it, making it a rich source of bioactive metabolites. Up to this end, the rhizosphere of Senna spectabilis, holds a clear cytotoxic potential, poorly studied regarding its chemical composition as well as any microbial interaction an thus will be the object of study for the application of these methodologies.