Production of 1,3-propanediol by Klebsiella pneumoniae and Escherichia coli: Media optimization and cloning of genes.

• Grant number: 11/01974-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2011
• End date: March 31, 2015
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Jonas Contiero
• Grantee: Paulo Marcelo Avila Neto
• Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
• Associated scholarship(s): 11/21620-1 - Cloning and expression of DHA genes from Klebsiella pneumoniae in Escherichia coli strains to 1,3-propanediol production, BE.EP.DR

Abstract

With the increasing use of biodiesel in the world, there was a large surplus of glycerol in the market. In Brazil, a market 200,000 tons of biodiesel glycerol is expected with the use of 5 % biodiesel to diesel . Nevertheless, the clarification and purification of this glycerol is still a very costly process , and the use of glycerol in raw form, and generate high value-added products, can help reduce the amount of biodiesel on the market, since storage costs or disposal of the crude glycerol is linked to the price of biodiesel. The biodiesel glycerol is can be used for the production of 1,3 -propanediol by bacteria. This design aims to clone and express genes related to the production of 1,3 -propanediol in E.coli strains in order to improve the production of that molecule .

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

AVILA NETO, Paulo Marcelo. Produção de 1,3-propanodiol por Klebsiella pneumoniae e Escherichia coli: otimização de meio e clonagem de genes. 2015. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Rio Claro Rio Claro.