Obtaining and analysis of enzyme lipase from the fungus Fusarium sp., varying the carbon source in submerged fermentation

• Grant number: 11/06671-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2011
• End date: December 31, 2011
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Valéria Marta Gomes de Lima
• Grantee: Carolina Scauri
• Host Institution: Faculdade de Ciências e Letras (FCL-ASSIS). Universidade Estadual Paulista (UNESP). Campus de Assis. Assis , SP, Brazil

Abstract

Lipases are enzymes that hydrolyze ester linkages of trialcilglicerois (GAD) also catalyze reactions of esterification and transesterification. These enzymes have great potential for application in various industries, the extensive applicability has led to a significant increase in recent decades, the industrial production of lipase. This high demand requires that the methods of mass production are carried out efficiently and affordably. The use of microorganisms in the production of lipase is a viable alternative for both has been studied extensively the media and culture conditions. To optimize the enzyme production and also find alternatives more economically attractive, many sources of carbon has been studied, because the lipid substrates act as inducers on lipase production in many species of fungi. Therefore, this paper will examine the conditions for more efficient production of lipase from Fusarium sp., in eight carbon sources, which were olive, crambe, corn, soybean, chicken, palm oil, linseed oil and glycerol. The cultivation of the strain of Fusarium sp. will be done in submerged fermentation and will be studied parameters that provide the obtain of enzyme with maximum activity, as a carbon source, pH and incubation time. (AU)