Development of a Lab-on-a-chip for glutamate analysis in Foodstuffs

• Grant number: 11/02477-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2012
• End date: February 29, 2016
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: José Alberto Fracassi da Silva
• Grantee: Camila Dalben Madeira Campos
• Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 08/53868-0 - Synthesis and chacterization of novel materials strategically structured and organized to be applied in analytical and photonic devices, AP.TEM
• Associated scholarship(s): 13/06625-2 - Development of enzymatic microdevices for glutamate analysis in complex matrixes, BE.EP.DR

Abstract

Glutamic Acid is constituent of many proteins, being important as neurotransmitter and in sugar and fat metabolism. Monosodic glutamate is one of the main responsible for Umami taste, usually associated to proteic food. It can be detected using capillary electrophoresis, among other techniques. In this project we intend to use the know how of mesoscale analysis and miniaturization techniques, used in medical analysis, to develop a lab-on-a-chip able to detect and quantify glutamate present in food. To reach this objective, there will be also necessary to miniaturize the sample pre-treatment. If successful, the device will permit fast and trustful detection with small sample amounts.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAMILA D. M. CAMPOS; RICHARD P. S. DE CAMPOS; JOSÉ A. FRACASSI DA SILVA; DOSIL P. JESUS; RICARDO M. ORLANDO. PREPARO DE AMOSTRAS ASSISTIDO POR CAMPO ELÉTRICO: FUNDAMENTOS, AVANÇOS, APLICAÇÕES E TENDÊNCIAS. Química Nova, v. 38, n. 8, p. 1093-1106, 2015-09-00. (11/02477-3, 13/22485-6)

PARK, J. K.; CAMPOS, C. D. M.; NEUZIL, P.; ABELMANN, L.; GUIJT, R. M.; MANZ, A.. Direct coupling of a free-flow isotachophoresis (FFITP) device with electrospray ionization mass spectrometry (ESI- MS). LAB ON A CHIP, v. 15, n. 17, p. 3495-3502, 2015. (13/06625-2, 11/02477-3)

MADEIRA CAMPOS, CAMILA DALBEN; DE CAMPOS BRAGA, PATRICIA APARECIDA; REYES REYES, FELIX GUILLERMO; FRACASSI DA SILVA, JOSE ALBERTO. Elimination of the artefact peaks in capillary electrophoresis determination of glutamate by using organic solvents in sample preparation. JOURNAL OF SEPARATION SCIENCE, v. 38, n. 21, p. 3781-3787, NOV 2015. (11/02477-3)

CAMPOS, C. D. M.; PARK, J. K.; NEUZIL, P.; DA SILVA, J. A. F.; MANZ, A.. Membrane-free electroextraction using an aqueous two-phase system. RSC ADVANCES, v. 4, n. 90, p. 49485-49490, 2014. (13/06625-2, 11/02477-3)

CAMPOS, CAMILA D. M.; DA SILVA, JOSE A. F.. Applications of autonomous microfluidic systems in environmental monitoring. RSC ADVANCES, v. 3, n. 40, p. 18216-18227, 2013. (11/02477-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CAMPOS, Camila Dalben Madeira. Development of a lab-on-a-chip for glutamate determination in food. 2016. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Química Campinas, SP.