Study of factors involved in DNA fragmentation of spermatozoa in ram.

• Grant number: 11/11231-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: September 01, 2011
• End date: September 30, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Mayra Elena Ortiz D' Avila Assumpcao
• Grantee: Thais Rose dos Santos Hamilton
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 13/05025-1 - Measurement of antioxidant enzymes in the ram's semen: cellular and plasma concentrations, BE.EP.DR

Abstract

DNA modifications can provoke changes in reproduction related processes, and the sperm chromatin integrity guarantees the delivery of paternal genetic information effectively. DNA injuries could be due to factors such as failure protamination, apoptosis and result from reactive oxygen species activity. This DNA damage in sperm can cause male infertility. There is little information on fragmentation rates and the correlation between sperm DNA fragmentation and male reproductive efficiency in ram. This work will be undertaken to evaluate the DNA fragmentation rate, type of protamine present in sperm and establish a correlation between the type of protamine and the sperm DNA fragmentation rate in ram. For this purpose we will determine the gene expression of protamine 1 and 2, and the transition proteins 1 and 2, and evaluate the susceptibility of sperm DNA fragmentation, as well as the its correlation to oxidative stress, apoptosis and packaging sperm chromatin damage.

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DOS SANTOS HAMILTON, THAIS ROSE; PEREZ SIQUEIRA, ADRIANO FELIPE; DE CASTRO, LETICIA SIGNORI; MENDES, CAMILLA MOTA; DELGADO, JULIANA DE CARVALHO; DE ASSIS, PATRICIA MONKEN; MESQUITA, LEONARDO PEREIRA; MAIORKA, PAULO CESAR; NICHI, MARCILIO; GOISSIS, MARCELO DEMARCHI; et al. Effect of Heat Stress on Sperm DNA: Protamine Assessment in Ram Spermatozoa and Testicle. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2018. (11/11231-8, 13/05025-1, 11/19551-1, 12/16157-3)

DOS SANTOS HAMILTON, THAIS ROSE; MENDES, CAMILLA MOTA; DE CASTRO, LETICIA SIGNORI; DE ASSIS, PATRICIA MONKEN; PEREZ SIQUEIRA, ADRIANO FELIPE; DELGADO, JULIANA DE CARVALHO; GOISSIS, MARCELO DEMARCHI; MUINO-BLANCO, TERESA; ALVARO CEBRIAN-PEREZ, JOSE; NICHI, MARCILIO; et al. Evaluation of Lasting Effects of Heat Stress on Sperm Profile and Oxidative Status of Ram Semen and Epididymal Sperm. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2016. (13/05025-1, 11/11231-8)

DOS SANTOS HAMILTON, THAIS ROSE; DUARTE, GABRIELA ESTEVES; VISINTIN, JOSE ANTONIO; ORTIZ D'AVILA ASSUMPCAO, MAYRA ELENA. Immunolocalization of antioxidant enzymes in testis of rams submitted to long-term heat stress. ZYGOTE, v. 27, n. 6, p. 432-435, DEC 2019. (11/11231-8)

DOS SANTOS HAMILTON, THAIS ROSE; DE CASTRO, LETICIA SIGNORI; DELGADO, JULIANA DE CARVALHO; DE ASSIS, PATRICIA MONKEN; PEREZ SIQUEIRA, ADRIANO FELIPE; MENDES, CAMILLA MOTA; GOISSIS, MARCELO DEMARCHI; MUINO-BLANCO, TERESA; CEBRIAN-PEREZ, JOSE ALVARO; NICHI, MARCILIO; et al. Induced lipid peroxidation in ram sperm: semen profile, DNA fragmentation and antioxidant status. Reproduction, v. 151, n. 4, p. 379-390, APR 2016. (13/05025-1, 11/11231-8)

DOS SANTOS HAMILTON, THAIS ROSE; PEREZ SIQUEIRA, ADRIANO FELIPE; DE CASTRO, LETICIA SIGNORI; MENDES, CAMILLA MOTA; DELGADO, JULIANA DE CARVALHO; DE ASSIS, PATRICIA MONKEN; MESQUITA, LEONARDO PEREIRA; MAIORKA, PAULO CESAR; NICHI, MARCILIO; GOISSIS, MARCELO DEMARCHI; et al. Effect of Heat Stress on Sperm DNA: Protamine Assessment in Ram Spermatozoa and Testicle. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, v. 2018, p. 14-pg., 2018-01-01. (12/16157-3, 13/05025-1, 11/11231-8, 11/19551-1)

DOS SANTOS HAMILTON, THAIS ROSE; MENDES, CAMILLA MOTA; DE CASTRO, LETICIA SIGNORI; DE ASSIS, PATRICIA MONKEN; PEREZ SIQUEIRA, ADRIANO FELIPE; DELGADO, JULIANA DE CARVALHO; GOISSIS, MARCELO DEMARCHI; MUINO-BLANCO, TERESA; ALVARO CEBRIAN-PEREZ, JOSE; NICHI, MARCILIO; et al. Evaluation of Lasting Effects of Heat Stress on Sperm Profile and Oxidative Status of Ram Semen and Epididymal Sperm. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, v. 2016, p. 12-pg., 2016-01-01. (13/05025-1, 11/11231-8)

DOS SANTOS HAMILTON, THAIS ROSE; SIMOES, RENATA; ORTIZ D'AVILA ASSUMPCAO, MAYRA ELENA. An improved acetic acid-urea polyacrylamide electrophoresis method to evaluate bovine sperm protamines. REPRODUCTION IN DOMESTIC ANIMALS, v. 56, n. 7, p. 1050-1056, JUL 2021. (11/11231-8, 10/09145-3)

RIBEIRO, LUANA VANESSA; DALCHIAVON, BARBARA ROST; ASSUMPAO, MAYRA ELENA ORTIZ D'AVILA; HAMILTON, THAIS ROSE DOS SANTOS. Immediate and late effects of long-term testicular heat stress on the number of seminiferous tubules and cellular content in Santa Ines rams. ANIMAL REPRODUCTION, v. 22, n. 4, p. 7-pg., 2025-01-01. (11/11231-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

HAMILTON, Thais Rose dos Santos. Study of factors involved in DNA fragmentation of spermatozoa in rams. 2014. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.