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THE STUDY OF THE EFFECTS OF THE TREATMENT WITH A SPECIF SERINOPROTEASE INHIBITOR ON THE PHYSIOPATHOLOGIC MECHANISMS IN THE PULMONARY EMPHYSEMA IN MURINES.

Grant number: 11/11979-2
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): December 01, 2011
Effective date (End): November 30, 2012
Field of knowledge:Health Sciences - Medicine - Medical Clinics
Principal researcher:Fernanda Degobbi Tenorio Quirino dos Santos Lopes
Grantee:Juliana Dias Lourenço
Home Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Chronic obstructive pulmonary disease (COPD) is the fourth leading cause of mortality worldwide and a major cause of chronic morbidity, causing significant disability and reduced quality of life for their patients and high costs to public health. In addition, there is no cure for COPD, and there are no therapies that effectively reduce its progression. Preliminary results obtained in our laboratory demonstrated that treatment with the use of BMTI, a specific protease inhibitor, attenuated the injury of the lung parenchyma in mice subjected to an experimental protocol of emphysema. To assess the effect of this inhibitor on the possible pathophysiological mechanisms involved in the development of emphysema may assist in future studies to treat this disease. Methods: C57BL / 6 (n = 32) will be submitted to a protocol of induction of pulmonary emphysema by intranasal instillation of 50 l of pig pancreatic elastase (0.667 IU) and one hour after, they will receive treatment with BMTI (35.54 pmol in 50 l, intranasal). After 21 days, will be collected bronchoalveolar lavage for the quantification of inflammatory cells. The lungs are removed for subsequent morphometric analysis after specific staining by immunohistochemistry method. Will be evaluated: number of macrophages, cells positive for MMP-12, caspase 3 and expression of 8-isoprostane in the parenchyma.