Polyphasic taxonomy of bacterial isolates from the composting of wastes from São Paulo Zoo

• Grant number: 11/06548-2
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: November 01, 2011
• End date: April 30, 2013
• Field of knowledge: Biological Sciences - Microbiology
• Principal Investigator: Luiz Juliano Neto
• Grantee: Patrícia Locosque Ramos
• Host Institution: Instituto Nacional de Farmacologia (INFAR). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

There are little explored in Brazil environments that can be described as sources of biodiversity with potential application in biotechnological processes, which in this context fits into the biomass generated in the composting of animal manure and vegetable waste produced at the Zoo of São Paulo (FPZSP) . Microorganisms have a high genetic diversity with unique functions and essential in maintaining the ecosystem. There is great expectation that the contribution of these micro-organisms can offer major advances in biotechnological processes, with Brazil being the holder of approximately 20% of all microbial diversity of the world, possessing one of the richest biomes in biodiversity - the Brazilian Atlantic Forest - which has been extensively studied. The microbial diversity of this area is vast and it is estimated that 99% of existing species are not yet identified. The taxonomic approach to material from composting has been developed in various locations, showing that in recent years, composting has attracted attention as an environmentally sensitive and viable alternative in the treatment of organic waste in the world. In this context, the polyphasic taxonomic identification of bacteria becomes crucial, since the FPZSP collects biological material originating in Brazil's biodiversity, which is surrounded by the Atlantic. This study will be based on techniques such as polyphasic taxonomy: Maldi-TOF sequencing of housekeeping genes and 16S rRNA, phenotypic tests and microbiological Amplified Fragment Length Polymorphism (AFLP) and DNA-DNA hybridization. Aiming thus creating the culture collection of routines FPZSP and implementation of methodologies for taxonomic classification of micro-organisms in the composting process (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RAMOS, PATRICIA L.; KONDO, MARCIA Y.; SANTOS, SAARA M. B.; DE VASCONCELLOS, SUZAN P.; ROCHA, RAFAEL C. S.; DA CRUZ, JOAO B.; EUGENIO, PATRICIA F. M.; CABRAL, HAMILTON; JULIANO, MARIA A.; JULIANO, LUIZ; et al. A Tropical Composting Operation Unit at SAo Paulo Zoo as a Source of Bacterial Proteolytic Enzymes. Applied Biochemistry and Biotechnology, v. 187, n. 1, p. 282-297, JAN 2019. (14/07037-0, 12/50191-4, 13/12106-8, 11/06548-2)