Hemicellulases and accessory proteins of filamentous fungi and actinomycetes for deconstruction of lignocellulosic biomass

• Grant number: 11/10834-0
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: December 01, 2011
• End date: April 30, 2015
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Gabriel Padilla
• Grantee: Diogo Robl
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The use of lignocellulosic materials can significantly reduce the cost of raw materials for ethanol production. However, in nature the lignocellulosic materials are degraded by a consortium of microorganisms that synthesize various hydrolytic enzymes able to loosen and degrade these materials.Therefore, formulations of enzyme cocktails for degradation of lignocellulosic materials has been widely studied. In the kingdom, Fungi several species have been characterized as hydrolytic enzymes producers such as pectinases, cellulases, xylanases and ²-glucosidases. In addition, filamentous bacteria such as actinomycetes produce hydrolytic enzymes of industrial interest such as cellulases and hemicellulases of high stability. Thus, this project aims to obtain an enzyme cocktail of hemicellulases and accessory proteins from filamentous fungi and actinomycetes, for the deconstruction of lignocellulosic biomass. A glicohidrolases screening will be performed from a fungi collection of the Laboratory of Microbiology and Molecular Biology (LabMicro), Universidade Federal do Paraná (UFPR) and an endophytic bacteria collection of ICB/USP. The best strains will be selected and their growth kinetics and enzyme production determined. The enzyme complexes will be analyzed by proteomics and will have hydrolysis optimized. In this way, enzymatic complexes blends will be developed with cellulolytic cocktails in order to enable the biomass saccharification to produce ethanol. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DIOGO ROBL; CARLA MONTANARI MERGEL; PATRICIA DOS SANTOS COSTA; JOSÉ GERALDO DA CRUZ PRADELLA; GABRIEL PADILLA. Endophytic Actinomycetes as Potential Producers of Hemicellulases and Related Enzymes for Plant Biomass Degradation. Brazilian Archives of Biology and Technology, v. 62, 2019-08-29. (11/10834-0)

DIOGO ROBL; VANIA VICENTE; IDA CHAPAVAL PIMENTEL; GABRIEL PADILLA; JOSÉ GERALDO DA CRUZ PRADELLA. Unveiling Xylanolytic Enzymes Production of Talaromyces wortmannii DR49 on Industrial Agro Wastes. Brazilian Archives of Biology and Technology, v. 64, 2022-02-21. (11/10834-0)