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Importance of the relation of the fatty acids omega-6/omega-3 in the feeding

Grant number: 11/18671-3
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): December 01, 2011
Effective date (End): November 30, 2012
Field of knowledge:Agronomical Sciences - Food Science and Technology
Principal Investigator:Monica Roberta Mazalli
Grantee:Juliana Messias Marques
Home Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
Associated research grant:09/14656-0 - Importance of the relation of the fatty acids ômega-6/ômega-3 in the feeding, AP.JP


The experiment will be carried through in the sector of Experimental of Rabbits young rabbits in growth will be used, of the New race Zelândia, kept in individual cages during all the experiment. The biological assay will be executed in a period of 240 days, divided in two phases. Phase 1 will correspond the 120 days of study, using rabbits submitted to the following diets: hipercholesterolaemic induction (0.5% of cholesterol), disequilibrium in the ratio of Omega-6/ômega-3 (n-6/n-3) of 15:1 and 1:15, respectively, and ratio of n-6/n-3 of 4:1 (control diet). In the Phase 16 assays will be formed, which will be divided randomly in 6 groups of 8 rabbits each. Sunflower Oil and flaxseed oil will be used as fatty acid sources of polyunsaturateds n-6 and n-3, respectively. Phase 2 will correspond of 120 days of study, sequential to Phase 1 (without interruption). The remaining animals will continue receiving the same diets from Phase 1, except in group B with hipercholesterolaemic induction that will receive beyond this last the control treatment to verify the effect. Monthly, it will have collection of blood of the rabbits for the total cholesterol determination, LDL and HDL-cholesterol and triacylglicerol. In the end of Phase 1, four animals of each group will be sacrificed with the removal aorta for posterior determination of lipids plates and oxidated LDL-cholesterol. Dosage of TBARS (Thiobarbituric Acid-Reactive Substances) will be used as index of oxidation of the LDL-cholesterol. In the end of the experiment after 240 days, all the rabbits will be abated and its aortas will be removed for more planimetric determination of lipids plates, index of oxidation of the LDL-cholesterol and verification if the supply of the control diet (ratio of n-6/n-3 of 4:1) will go to interrupt or inhibit the atherosclerotic injuries. Also in will be developed methodology "in vitro" to evaluate the best relation of fatty acid n-6/ n-3 in the prevention of the development of atherosclerosis and implications theses fatty acids in the endothelial, hepatic and adipose cells. Moreover, the activity of proteins and the expression of genes will be investigated involved in the etiology of atherosclerosis.