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Effects of different polishing techniques on the adherence of Streptococcus mutans on the surface of restorative composites and glass ionomer sealant.

Grant number: 11/06419-8
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): December 01, 2011
Effective date (End): November 30, 2012
Field of knowledge:Health Sciences - Dentistry
Principal researcher:Marcelo Ferrarezi de Andrade
Grantee:Flávia Yukie Sakomura
Home Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil


The objective of this study is to analyze the adhesion in vitro of Streptococcus mutans in different types of composite resins and a new glass ionomer cement modified by resin, after being subjected to different finishing and polishing techniques. Using a metal matrix (10 mm diameter and 2 mm thick) bodies will be made 10-of-test (n = 10) per group: FiltekTM P90 (3M ESPE), FiltekTM Z350XT (3M ESPE) and ionomer sealant ClimproTM Varnish XT (3M ESPE), which will be submitted to different finishing and polishing techniques: polyester strips (control), aluminum oxide disks Sof-Lex Pop On ", rubber tips and multilayered abrasive FlexiCups - Cosmedent, totaling 120 the specimens. The polymerization is carried out using the LED device Celalux ® (Voco) with power density of 776 mW / cm ² for 40 seconds. The samples will be stored in distilled water at 37 ° C ± 1 ° C for 24 hours, to then be subjected to surface treatment, and then be immersed in a culture of Streptococcus mutans grown in Petri dishes containing the medium Mitis Salivarius sucrose. Then, the plates will be taken to a bacteriological incubator at 37 ° C for 24 h to occur adherence of microorganisms. To evaluate the adherence of S. mutans to the specimens, will be used by scanning laser confocal microscope (FluoView FV1000, Olympus, Japan), and the results are statistically analyzed by analysis of variance (ANOVA) and Tukey's test, with significance level of 5% (p > 0.05).