Production of cellulolytic enzymes associated and growth kinetics of fungi Trichoderma reesei and Thermoascus aurantiacus in solid state fermentation

• Grant number: 11/21239-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2012
• End date: December 31, 2012
• Field of knowledge: Biological Sciences - Biochemistry - Biochemistry of Microorganisms
• Principal Investigator: Roberto da Silva
• Grantee: Priscila Aparecida Casciatori
• Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil

Abstract

At this time of discussion of country's energy matrix, the cellulases are relevant for the hydrolysis of lignocellulosic materials aimed at producing second-generation ethanol. Although the solid-state fermentation (SSF) to produce high concentrations of enzymes, this technique still lacks scientific and technological knowledge that allows your application on a large scale for different combinations of microorganisms and substrates, with a focus on filamentous fungi as agents of fermentation and agro-industrial solid wastes as substrates economical and environmentally friendly. In this context, the objective of this project is to study the production of cellulolytic enzymes and the kinetics of biomass growth potential of two fungi producing cellulase in a substrate composed of wheat bran and sugar cane bagasse, predominantly from bagasse. The fungi to be used will be the mesophilic Trichoderma reesei and thermophilic Thermoascus aurantiacus. The use of fungi with different thermal preferences lends itself to compare the effect of temperature on enzyme production and the growth kinetics. Measurement techniques will be used for indirect assessment of growth, two techniques biomarkers (total protein and glucosamine content of the fermented material) and a respirometric technique (carbon dioxide production). The experimental data are fitted by kinetic equations (first order, logistics and others) from what will be possible to estimate the specific growth rate of the fungus, which is an important and not available in the literature for the fungi studied here. Enzyme activity and kinetics of cellulase production will be evaluated using measures of activities of filter paper and betaglicosidase. For this purpose, scale fermentation tests of bottles will be conducted simultaneously and independently, in order to speed up data retrieval. The project is part of the theme of FAPESP Bioenergy Research (BIOEN) and the Paulista Center for Bioenergy Research and is being proposed along the line of research Bioenergy and Utilization of Waste Treatment from the Graduate Program in Chemistry of the Institute of Biosciences, Letters and Exact Sciences in Sao Jose do Rio Preto. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FRASSATTO, PRISCILA APARECIDA CASCIATORI; CASCIATORI, FERNANDA PERPETUA; THOMEO, JOAO CLAUDIO; GOMES, ELENI; BOSCOLO, MAURICIO; DA SILVA, ROBERTO. Fungal cellulases: production by solid-state cultivation in packed-bed bioreactor using solid agro-industrial by-products as substrates and application for hydrolysis of sugarcane bagasse. SEMINA-CIENCIAS AGRARIAS, v. 41, n. 5, 1, p. 2097-2115, 2020. (13/01756-1, 18/00996-2, 17/16482-5, 11/07453-5, 10/12624-0, 11/21239-6)

CASCIATORI, FERNANDA PERPETUA; LAURENTINO, CARMEM LUCIA; LEAL DA COSTA, KAREN KRISTHINE; CASCIATORI, PRISCILA APARECIDA; THOMEO, JOAO CLAUDIO. HYGROSCOPIC PROPERTIES OF ORANGE PULP AND PEEL. JOURNAL OF FOOD PROCESS ENGINEERING, v. 36, n. 6, p. 803-810, DEC 2013. (12/13939-0, 11/07453-5, 10/12624-0, 11/21239-6)

FRASSATTO, PRISCILA APARECIDA CASCIATORI; CASCIATORI, FERNANDA PERPETUA; THOMEO, JOAO CLAUDIO; GOMES, ELENI; BOSCOLO, MAURICIO; DA SILVA, ROBERTO. Fungal cellulases: production by solid-state cultivation in packed-bed bioreactor using solid agro-industrial by-products as substrates and application for hydrolysis of sugarcane bagasse. SEMINA-CIENCIAS AGRARIAS, v. 41, n. 5, p. 19-pg., 2020-01-01. (17/16482-5, 11/07453-5, 13/01756-1, 10/12624-0, 18/00996-2, 11/21239-6)