Several studies using inhibitory avoidance models have demonstrated the importance of limbic structures as the hippocampus, amygdala and medial prefrontal cortex (mPFC) on emotional memory. But there are few studies in which the model of inhibitory avoidance of the step down was used to assess the involvement of these limbic structures in the emotional memory of mice. Given that our goals are: Experiment 1 - verify the role of the histaminergic system in limbic structures as the hippocampus, amygdala and mPFC by RT-PCR (polymerase chain reaction in real time) in mice submitted to inhibitory avoidance model type step down; Experiment 2 - to investigate the role of the hippocampus, amygdala and mPFC, through the administration of anisomycin (ANI) (the drug that inhibits protein synthesis), on modulation of emotional memory in mice subjected to the step down model . If the experiment proves a gene expression, histamine antagonists injected in the areas will be studied in a third experiment. For this, 90 mice of Swiss-Albino strain, weighing between 25 and 35 grams will be used. Experiment 1 will consist of 30 animals assigned into 3 groups: control (n = 10), which subjects are not tested for step down and will be the analysis of gene expression of HDC (histidine decarboxylase) and receptors H1, H2 and H3 by RT-PCR (polymerase chain reaction in real time) after training (n = 10) and Post-Test (n = 10), which the subjects will be exposed to the test inhibitory avoidance and will be done the analysis of gene expression (RT-PCR). Experiment 2 will consist of 60 animals divided into three groups: HD (n = 20), animals that are treated with ANI or vehicle into the dorsal hippocampus half hour before training, AMD (n = 20), animals that are treated with ANI or vehicle in the amygdala half hour before training; mPFC (n = 20), animals that are treated with ANI or vehicle into the medial prefrontal cortex half hour before training. Data from experiments will be analyzed by one-way ANOVA (Experiment 1) or two-way repeated measures (factor 1: treatment, factor 2: exposure, Experiment 2), followed by Duncan's test (parametric). Values will be considered significant at p lower or equal to 0.05.
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