Involvement of histaminergic system in limbic structures on the memory of inhibitory avoidance in mice

• Grant number: 11/19472-4
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: February 01, 2012
• End date: October 31, 2015
• Field of knowledge: Humanities - Psychology - Physiological Psychology
• Principal Investigator: Rosana Mattioli
• Grantee: Lucas Canto de Souza
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated scholarship(s): 12/05838-0 - The evaluation between histaminergic and cholinergic neurotransmission on object recognition and emotional memory, BE.EP.DR

Abstract

Several studies using inhibitory avoidance models have demonstrated the importance of limbic structures as the hippocampus, amygdala and medial prefrontal cortex (mPFC) on emotional memory. But there are few studies in which the model of inhibitory avoidance of the step down was used to assess the involvement of these limbic structures in the emotional memory of mice. Given that our goals are: Experiment 1 - verify the role of the histaminergic system in limbic structures as the hippocampus, amygdala and mPFC by RT-PCR (polymerase chain reaction in real time) in mice submitted to inhibitory avoidance model type step down; Experiment 2 - to investigate the role of the hippocampus, amygdala and mPFC, through the administration of anisomycin (ANI) (the drug that inhibits protein synthesis), on modulation of emotional memory in mice subjected to the step down model . If the experiment proves a gene expression, histamine antagonists injected in the areas will be studied in a third experiment. For this, 90 mice of Swiss-Albino strain, weighing between 25 and 35 grams will be used. Experiment 1 will consist of 30 animals assigned into 3 groups: control (n = 10), which subjects are not tested for step down and will be the analysis of gene expression of HDC (histidine decarboxylase) and receptors H1, H2 and H3 by RT-PCR (polymerase chain reaction in real time) after training (n = 10) and Post-Test (n = 10), which the subjects will be exposed to the test inhibitory avoidance and will be done the analysis of gene expression (RT-PCR). Experiment 2 will consist of 60 animals divided into three groups: HD (n = 20), animals that are treated with ANI or vehicle into the dorsal hippocampus half hour before training, AMD (n = 20), animals that are treated with ANI or vehicle in the amygdala half hour before training; mPFC (n = 20), animals that are treated with ANI or vehicle into the medial prefrontal cortex half hour before training. Data from experiments will be analyzed by one-way ANOVA (Experiment 1) or two-way repeated measures (factor 1: treatment, factor 2: exposure, Experiment 2), followed by Duncan's test (parametric). Values will be considered significant at p lower or equal to 0.05.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BAPTISTA-DE-SOUZA, DANIELA; RODRIGUES TAVARES, LIGIA RENATA; FURUYA-DA-CUNHA, ELKE MAYUMI; CARNEIRO DE OLIVEIRA, PAULO EDUARDO; CANTO-DE-SOUZA, LUCAS; NUNES-DE-SOUZA, RICARDO LUIZ; CANTO-DE-SOUZA, AZAIR. Chronic Fluoxetine Impairs the Effects of 5-HT1A and 5-HT2C Receptors Activation in the PAG and Amygdala on Antinociception Induced by Aversive Situation in Mice. FRONTIERS IN PHARMACOLOGY, v. 11, MAR 11 2020. (11/19472-4)

CANTO-DE-SOUZA, L.; MATTIOLI, R.. The consolidation of inhibitory avoidance memory in mice depends on the intensity of the aversive stimulus: The involvement of the amygdala, dorsal hippocampus and medial prefrontal cortex. NEUROBIOLOGY OF LEARNING AND MEMORY, v. 130, p. 44-51, APR 2016. (11/19472-4)

BAPTISTA-DE-SOUZA, DANIELA; PELARIN, VINICIUS; CANTO-DE-SOUZA, LUCAS; NUNES-DE-SOUZA, RICARDO LUIZ; CANTO-DE-SOUZA, AZAIR. Interplay between 5-HT2C and 5-HT1A receptors in the dorsal periaqueductal gray in the modulation of fear-induced antinociception in mice. Neuropharmacology, v. 140, p. 100-106, SEP 15 2018. (10/06654-4, 11/19472-4, 09/17938-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SOUZA, Lucas Canto de. Involvement of histaminergic system in limbic structures on the memory of inhibitory avoidance in mice. 2015. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (PCARP/BC) Ribeirão Preto.