Use of peroxidase in the identification and varietal determination in soybean (Glycine max (L.) Merr.) seeds

• Grant number: 11/04567-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2012
• End date: February 28, 2013
• Field of knowledge: Agronomical Sciences - Agronomy - Crop Science
• Principal Investigator: Edvaldo Aparecido Amaral da Silva
• Grantee: Bárbara Panoff Valário
• Host Institution: Faculdade de Ciências Agronômicas (FCA). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

With the increase of the number to cultivate of soy and with the little genetic difference between them, is each more difficult time the identification to cultivate and the verification of varietal contamination for the method of peroxidase. It is through tests in laboratory among others more modern techniques, that if can detect the different characteristics cultivating between them. Peroxidases also has the function of catalizar oxidativas reactions and uses hydrogen peroxide (H2O2) as the aceptor of electrons. To cultivate them of soy are separate in two groups on the basis of the activity, high or low, of peroxidase in the tegumento. After the accomplishment of genetic analysis, told that such activity is controlled for a dominant gene. High activity of the presence of, at least, one alelo dominant (EpEp or Epep), whereas low activity results of the presence of the recessivo pair (epep). The use of molecular markers in the identification of varieties of soy is of great importance not to inside have more variations between positive and negative reactions of the same variety, becoming the colorimétrico test, that is the available methodology currently, sufficiently doubtful. In this context, the general objective of the work is to make possible the technique of molecular biology to facilitate to the identification of varieties of soy through the enzyme peroxidase. The study it will be lead in the Department of Vegetal Production of the College of Agronômicas/Unesp Sciences, Campus of Botucatu-SP. The traditional and compared colorimétrico test with the results found in the PCR will be carried through. 13 will be used to cultivate of importance, of these, will be preselected 6 will cultivate with positive reaction to peroxidase: BRS 320, BRS 284, BRS 232, BRS 7860RR, BRSMG 760SRR, BRS295RR, 4 to cultivate refusals to peroxidase: BRS 326, BRS 8160RR, BRSMG 800ª, BRS Valuable RR and 3 to cultivate with positive and negative reaction: BRSGO 8060, BRS 270RR and BRS 239. In parallel to the colorimétrico test, the DNA will be extracted of the tegumento of the seeds, primers will be drawn, followed of the reaction of PCR and eletroforese in gel of agarose. The presence or absence of bands in the gel will indicate positive or negative reaction for peroxidase.