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Establishment of a protocol to control expression of endophytic bacteria in the process of in vitro multiplication of eucalyptus

Grant number: 11/15861-6
Support type:Scholarships in Brazil - Master
Effective date (Start): March 01, 2012
Effective date (End): May 31, 2013
Field of knowledge:Biological Sciences - Botany - Pant Physiology
Principal Investigator:Marcilio de Almeida
Grantee:Gabriela Ferraz Leone
Home Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil


One of the biggest problems detected during the processes of plant micropropagation is the contamination of culture medium resulting from inadequate disinfestation, usually observed within 15 days after explants inoculation. The removal of the epiphytic microbiota is essential to obtain healthy seedlings. However, it is much common microorganisms occurrence after the establishment of seedlings, resulting in discard of numerous commercial individuals with high value. However, can to consider that manifestation is characterized as responses of the endophytic microbiota that colonize plant tissues, the possible physical or nutritional stresses that involve the environment in vitro. Showing that this presence is a result of succession of bacterial species, which remain in the explants used as a source of clonal multiplication. A good result for the control of bacterial expression in vitro in an attempt to keep the stuff in development, is the aplication of antibiotic treatment in the culture medium. In this context, the main objective of this project is to establish efficient protocols for the manifestation of chemical control of endophytic bacteria in the process of micropropagation of Eucalyptus, by testing for antibiotic therapy, and to identify any differences between the niches for colonization of explants sources (crown's megaestaca and girdling of the base), for histological and molecular techniques (DGGE).

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LEONE, GABRIELA FERRAZ; MAIA ANDRADE, PEDRO AVELINO; DE ALMEIDA, CAROLINA VIEIRA; DE ALMEIDA, CRISTINA VIEIRA; ANDREOTE, FERNANDO DINI; DE ALMEIDA, MARCILIO. Use of antibiotics to control endophytic bacterial growth migration onto culture medium in Eucalyptus cloeziana F.Muell.: a micropropagation approach. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, v. 55, n. 4, p. 421-432, AUG 2019. Web of Science Citations: 0.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
LEONE, Gabriela Ferraz. Estabelecimento de protocolo para controlar a manifestação de bactérias endofíticas no processo de multiplicação in vitro de eucalipto. 2013. Master's Dissertation - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz Piracicaba.

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