Study of apoptosis regulatory proteins expression in an in vitro model of in situ like malignant neoplastic areas.

• Grant number: 11/21157-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2012
• End date: February 28, 2014
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Vera Cavalcanti de Araujo
• Grantee: Carolina Amália Barcellos Silva
• Host Institution: Centro de Pesquisas Odontológicas São Leopoldo Mandic. Faculdade São Leopoldo Mandic (SLMANDIC). Sociedade Regional de Ensino e Saúde S/S Ltda (SRES). Campinas , SP, Brazil

Abstract

Several studies have demonstrated the role of myoepithelial cells in the behavior of salivary glands and breast tumors. In salivary glands tumors, these cells are associated with better prognosis of these tumors, and in breast cancer, their function is related, mainly, with the progression of an in situ to invasive carcinoma. These studies revealed the tumor suppressor role of these cells, promoting defense against cancer progression. In this tumorl microenvironment, the study of the imbalance among the regulatory pathways of the proliferation control and apoptosis, in the malignant cells such as in the myoepithelial cells, may contribute for the better understanding of the tumoral progression. Abnormal expression of the apoptosis regulatory proteins has been reported in several human cancers, modulating the cancer invasion and progression. Thus, the aim of this research is to study the expression of the regulatory apoptosis proteins, Bcl-2, Bax 1 and Surviving in in situ like neoplastic areas when malignant cells from oral squamous cell carcinoma are surrounded by myoepithelial cells from pleomorphic adenoma, in different periods of cell culture, in order to identify the moment and role of these proteins expression in the regulation of the neoplastic process. For this, the myoepithelial cells from pleomorphic adenoma will be cultured, adding malignant conditioned medium from oral squamous cell carcinoma, under the influence of a matrix protein, the fibronectin. In addition, the expression of apoptosis regulatory proteins (Bcl-2, Bax 1 and Survivin) in in situ like neoplastic areas, in different periods of cell culture, will be evaluate by indirect immunofluorescence and the quantitative analysis of these proteins will be performed by quantitative polymerase chain reaction.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MORAES, PAULO DE CAMARGO; BARCELLOS SILVA, CAROLINA AMALIA; SOARES, ANDRESA BORGES; PASSADOR-SANTOS, FABRICIO; PIZZIGATTI CORREA, MARIA ELVIRA; DE ARAUJO, NEY SOARES; DE ARAUJO, VERA CAVALCANTI. Tooth alterations in areas of bisphosphonate-induced osteonecrosis. CLINICAL ORAL INVESTIGATIONS, v. 19, n. 2, p. 489-495, MAR 2015. (11/21157-0)

BARCELLOS SILVA, CAROLINA AMALIA; MARTINEZ, ELIZABETH FERREIRA; DIAS DEMASI, ANA PAULA; ALTEMANI, ALBINA; DA SILVEIRA BOSSONARO, JERUZA PINHEIRO; ARAUJO, NEY SOARES; DE ARAUJO, VERA CAVALCANTI. Cellular senescence and autophagy of myoepithelial cells are involved in the progression of in situ areas of carcinoma ex-pleomorphic adenoma to invasive carcinoma. An in vitro model. JOURNAL OF CELL COMMUNICATION AND SIGNALING, v. 9, n. 3, p. 255-265, SEP 2015. (11/21157-0)