Evaluation of transcription factors that induce ephitelial-mesenchymal transition (EMT) in endothelial cell biology

• Grant number: 11/21740-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2012
• End date: July 31, 2015
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Simone Kashima Haddad
• Grantee: Mariana Tomazini Pinto
• Host Institution: Hemocentro de Ribeirão Preto. Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto da USP (HCMRP). Secretaria da Saúde (São Paulo - Estado). Ribeirão Preto , SP, Brazil

Abstract

The Endothelial Mesenchymal Transition (EndMT) is categorized as a specialized form of Epithelial Mesenchymal transition (EMT), in which endothelial cells (CEs) lose intracellular junctions and endothelial markers, acquire mesenchyal phenotype, as well as invasive and migratory functions. In physiological condition, the EndMT is envolved in embryogenesis and in the origin and maintenance of mesenchymal stem cells (MSCs) in the adult organism. In pathological condition, the EndMT is responsible for the fibrosis formation and participates in tumor progression. Regarding the mechanisms of induction of EndMT, it is postulated that EMT and EndMT share the same molecular mechanisms. Several transcription factors are related to the induction and regulation of EMT. Thus, the objective of this study is to investigate EndMT process in CEs of different sources. For this purpose, transcription factors associated with EMT will be overexpressed in CEs and EndMT will be evaluated. Additionally, we will analyze which molecular factors are involved in this process. Understanding these mechanisms will allow a better understanding of CEs biology, as well as the EndMT function in the stem cell generation. Moreover, this study will contribute to new knowledge and development of new therapeutic approaches using cell therapy. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PINTO, MARIANA TOMAZINI; FERREIRA MELO, FERNANDA URSOLI; MALTA, TATHIANE MAISTRO; RODRIGUES, EVANDRA STRAZZA; PLACA, JESSICA RODRIGUES; JR SILVA, WILSON ARAUJO; PANEPUCCI, RODRIGO ALEXANDRE; COVAS, DIMAS TADEU; RODRIGUES, CLAUDIA DE OLIVEIRA; KASHIMA, SIMONE. Endothelial cells from different anatomical origin have distinct responses during SNAIL/TGF-beta 2-mediated endothelial-mesenchymal transition. AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH, v. 10, n. 12, p. 4065-4081, 2018. (13/08135-2, 11/21740-7)

PINTO, MARIANA T.; COVAS, DIMAS T.; KASHIMA, SIMONE; RODRIGUES, CLAUDIA O.. Endothelial Mesenchymal Transition: Comparative Analysis of Different Induction Methods. BIOLOGICAL PROCEDURES ONLINE, v. 18, APR 27 2016. (11/21740-7)

PINTO, MARIANA TOMAZINI; MALTA, TATHIANE MAISTRO; RODRIGUES, EVANDRA STRAZZA; PINHEIRO, DANIEL GUARIZ; PANEPUCCI, RODRIGO ALEXANDRE; RIBEIRO MALMEGRIM DE FARIAS, KELEN CRISTINA; SOUSA, ALESSANDRA DE PAULA; TAKAYANAGUI, OSVALDO MASSAITI; TANAKA, YUETSU; COVAS, DIMAS TADEU; et al. Genes Related to Antiviral Activity, Cell Migration, and Lysis Are Differentially Expressed in CD4(+) T Cells in Human T Cell Leukemia Virus Type 1-Associated Myelopathy/Tropical Spastic Paraparesis Patients. AIDS Research and Human Retroviruses, v. 30, n. 6, p. 610-622, JUN 2014. (11/21740-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PINTO, Mariana Tomazini. Evaluation of inducing factors of epithelial-mesenchymal transition (EMT) in the endothelial cells biology. 2015. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) Ribeirão Preto.