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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Genes Related to Antiviral Activity, Cell Migration, and Lysis Are Differentially Expressed in CD4(+) T Cells in Human T Cell Leukemia Virus Type 1-Associated Myelopathy/Tropical Spastic Paraparesis Patients

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Pinto, Mariana Tomazini [1, 2, 3] ; Malta, Tathiane Maistro [1, 2, 3] ; Rodrigues, Evandra Strazza [1, 2, 3] ; Pinheiro, Daniel Guariz [1, 2, 4] ; Panepucci, Rodrigo Alexandre [1, 2, 5] ; Ribeiro Malmegrim de Farias, Kelen Cristina [1, 2, 3] ; Sousa, Alessandra De Paula [1, 2] ; Takayanagui, Osvaldo Massaiti [5] ; Tanaka, Yuetsu [6] ; Covas, Dimas Tadeu [1, 2, 5] ; Kashima, Simone [1, 2, 3]
Total Authors: 11
[1] Natl Inst Sci & Technol Stem Cell & Cell Therapy, Ctr Cell Based Therapy, Ribeirao Preto - Brazil
[2] Natl Inst Sci & Technol Stem Cell & Cell Therapy, Reg Blood Ctr Ribeirao Preto, Ribeirao Preto - Brazil
[3] Univ Sao Paulo, Fac Pharmaceut Sci, BR-14049 Ribeirao Preto - Brazil
[4] Univ Sao Paulo, Dept Genet, Fac Med Ribeirao Preto, BR-14049 Ribeirao Preto - Brazil
[5] Univ Sao Paulo, Fac Med Ribeirao Preto, BR-14049 Ribeirao Preto - Brazil
[6] Univ Ryukyus, Grad Sch Med, Dept Immunol, Okinawa - Japan
Total Affiliations: 6
Document type: Journal article
Source: AIDS Research and Human Retroviruses; v. 30, n. 6, p. 610-622, JUN 2014.
Web of Science Citations: 3

Human T cell leukemia virus type 1 (HTLV-1) preferentially infects CD4(+) T cells and these cells play a central role in HTLV-1 infection. In this study, we investigated the global gene expression profile of circulating CD4(+) T cells from the distinct clinical status of HTLV-1-infected individuals in regard to TAX expression levels. CD4(+) T cells were isolated from asymptomatic HTLV-1 carrier (HAC) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients in order to identify genes involved in HAM/TSP development using a microarray technique. Hierarchical clustering analysis showed that healthy control (CT) and HTLV-1-infected samples clustered separately. We also observed that the HAC and HAM/TSP groups clustered separately regardless of TAX expression. The gene expression profile of CD4(+) T cells was compared among the CT, HAC, and HAM/TSP groups. The paxillin (Pxn), chemokine (C-X-C motif) receptor 4 (Cxcr4), interleukin 27 (IL27), and granzyme A (Gzma) genes were differentially expressed between the HAC and HAM/TSP groups, regardless of TAX expression. The perforin 1 (Prf1) and forkhead box P3 (Foxp3) genes were increased in the HAM/TSP group and presented a positive correlation to the expression of TAX and the proviral load (PVL). The frequency of CD4(+)FOXP3(+) regulatory T cells (Treg) was higher in HTLV-1-infected individuals. Foxp3 gene expression was positively correlated with cell lysis-related genes (Gzma, Gzmb, and Prf1). These findings suggest that CD4(+) T cell activity is distinct between the HAC and HAM/TSP groups. (AU)

FAPESP's process: 11/21740-7 - Evaluation of transcription factors that induce ephitelial-mesenchymal transition (EMT) in endothelial cell biology
Grantee:Mariana Tomazini Pinto
Support type: Scholarships in Brazil - Doctorate