Genes Related to Antiviral Activity, Cell Migration, and Lysis Are Differentially Expressed in CD4(+) T Cells in Human T Cell Leukemia Virus Type 1-Associated Myelopathy/Tropical Spastic Paraparesis Patients

Author(s):Show less -

Pinto, Mariana Tomazini ^{[1, 2, 3]} ; Malta, Tathiane Maistro ^{[1, 2, 3]} ; Rodrigues, Evandra Strazza ^{[1, 2, 3]} ; Pinheiro, Daniel Guariz ^{[1, 2, 4]} ; Panepucci, Rodrigo Alexandre ^{[1, 2, 5]} ; Ribeiro Malmegrim de Farias, Kelen Cristina ^{[1, 2, 3]} ; Sousa, Alessandra De Paula ^{[1, 2]} ; Takayanagui, Osvaldo Massaiti ^[5] ; Tanaka, Yuetsu ^[6] ; Covas, Dimas Tadeu ^{[1, 2, 5]} ; Kashima, Simone ^{[1, 2, 3]}

Affiliation

^[1] Natl Inst Sci & Technol Stem Cell & Cell Therapy, Ctr Cell Based Therapy, Ribeirao Preto - Brazil

^[2] Natl Inst Sci & Technol Stem Cell & Cell Therapy, Reg Blood Ctr Ribeirao Preto, Ribeirao Preto - Brazil

^[3] Univ Sao Paulo, Fac Pharmaceut Sci, BR-14049 Ribeirao Preto - Brazil

^[4] Univ Sao Paulo, Dept Genet, Fac Med Ribeirao Preto, BR-14049 Ribeirao Preto - Brazil

^[5] Univ Sao Paulo, Fac Med Ribeirao Preto, BR-14049 Ribeirao Preto - Brazil

^[6] Univ Ryukyus, Grad Sch Med, Dept Immunol, Okinawa - Japan

• Document type: Journal article
• Source: AIDS Research and Human Retroviruses; v. 30, n. 6, p. 610-622, JUN 2014.
• Web of Science Citations: 3

Abstract

Human T cell leukemia virus type 1 (HTLV-1) preferentially infects CD4(+) T cells and these cells play a central role in HTLV-1 infection. In this study, we investigated the global gene expression profile of circulating CD4(+) T cells from the distinct clinical status of HTLV-1-infected individuals in regard to TAX expression levels. CD4(+) T cells were isolated from asymptomatic HTLV-1 carrier (HAC) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients in order to identify genes involved in HAM/TSP development using a microarray technique. Hierarchical clustering analysis showed that healthy control (CT) and HTLV-1-infected samples clustered separately. We also observed that the HAC and HAM/TSP groups clustered separately regardless of TAX expression. The gene expression profile of CD4(+) T cells was compared among the CT, HAC, and HAM/TSP groups. The paxillin (Pxn), chemokine (C-X-C motif) receptor 4 (Cxcr4), interleukin 27 (IL27), and granzyme A (Gzma) genes were differentially expressed between the HAC and HAM/TSP groups, regardless of TAX expression. The perforin 1 (Prf1) and forkhead box P3 (Foxp3) genes were increased in the HAM/TSP group and presented a positive correlation to the expression of TAX and the proviral load (PVL). The frequency of CD4(+)FOXP3(+) regulatory T cells (Treg) was higher in HTLV-1-infected individuals. Foxp3 gene expression was positively correlated with cell lysis-related genes (Gzma, Gzmb, and Prf1). These findings suggest that CD4(+) T cell activity is distinct between the HAC and HAM/TSP groups. (AU)

• FAPESP's process: 11/21740-7 - Evaluation of transcription factors that induce ephitelial-mesenchymal transition (EMT) in endothelial cell biology
• Grantee: Mariana Tomazini Pinto
• Support type: Scholarships in Brazil - Doctorate