Research of encoding genes of staphylococcal enterotoxins and vancomycin resistance gene vanA: detection of genic expression in Staphylococcus coagulase positive and negative in bovine mastitis

• Grant number: 11/21142-2
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): March 01, 2012
• Effective date (End): February 28, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Helio Langoni
• Grantee: Felipe de Freitas Guimarães
• Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The concern of the genus Staphylococcus and importance have increased substantially, in animals and mainly in humans. Currently in addition to S. aureus, other coagulase-positive staphylococci (CPS) and coagulase-negative staphylococci (SCN) considered of minor importance, are attracting the interest of researchers because of the risk to public health represented by these micro-organisms in the etiology of mastitis in relation to antimicrobial resistance production capacity and heat-stable enterotoxin. The participation of these pathogens in bovine mastitis by milk and broadcasting justifies the need to investigate the toxigenic capacity and antimicrobial resistance. The objectives of this project: identify the main species, determine the potential risk to public health represented by the toxigenic capacity of strains and vancomycin resistance by detection of the vanA gene. It will be studied 150 strains of SCP and SCN 150 strains isolated from cases of bovine mastitis from different properties of the State of Sao Paulo. From the isolates will be analyzed for the presence of genes for production of enterotoxins by Polymerase Chain Reaction (PCR). Gene expression will be evaluated by Real-Time PCR (RTQ-PCR). The isolates will be subjected to sensitivity profile correlated with the detection of the vanA gene by PCR. The relevance of this project is based on the need to contribute to assessing the risk of mastitis by SCP and SCN in the food security of consumers of milk. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GUIMARAES, F. F.; MANZI, M. P.; JOAQUIM, S. F.; RICHINI-PEREIRA, V. B.; LANGONI, H. Short communication: Outbreak of methicillin-resistant Staphylococcus aureus (MRSA)-associated mastitis in a closed dairy herd. JOURNAL OF DAIRY SCIENCE, v. 100, n. 1, p. 726-730, JAN 2017. Web of Science Citations: 12.

FELIPE FREITAS GUIMARÃES; SAMEA FERNANDES JOAQUIM; MARCELA PINHO MANZI; RODRIGO COSTA DA SILVA; ARIANE CRISTINA MENDES DE OLIVEIRA BRUDER-NASCIMENTO; ELIZABETH OLIVEIRA COSTA; HÉLIO LANGONI. Comparison phenotypic and genotypic identification of Staphylococcus species isolated from bovine mastitis(1). Pesquisa Veterinária Brasileira, v. 36, n. 12, p. 1160-1164, Dez. 2016. Web of Science Citations: 0.

GUIMARAES, FELIPE DE FREITAS; NOBREGA, DIEGO BONIN; RICHINI-PEREIRA, VIRGINIA BODELAO; MARSON, PAMELA MERLO; DE FIGUEIREDO PANTOJA, JOSE CARLOS; LANGONI, HELIO. Enterotoxin genes in coagulase-negative and coagulase-positive staphylococci isolated from bovine milk. JOURNAL OF DAIRY SCIENCE, v. 96, n. 5, p. 2866-2872, MAY 2013. Web of Science Citations: 38.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

GUIMARÃES, Felipe de Freitas. Detecção de genes codificadores de enterotoxinas e resistência aos antimicrobianos fenotípica e genotípica, mecA e vanA e expressão gênica em estafilococos SCP e SCN isolados de mastite bovina. 2015. 127 f. Doctoral Thesis - Universidade Estadual Paulista "Júlio de Mesquita Filho" Faculdade de Medicina Veterinária..