Demography and genetic variation of the puma (Puma concolor, Felidae) in Conservation Units of Serra do Mar and Serra da Mantiqueira.

• Grant number: 12/00534-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2012
• End date: June 30, 2015
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: Pedro Manoel Galetti Junior
• Grantee: Andiara Silos Moraes de Castro e Souza
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil

Abstract

The increasing human intervention in the Atlantic Forest severely damaged the landscape, leaving only fragments of forest currently. The puma (Puma concolor) occurs in this biome and is threatened by the reduction and fragmentation of their habitats. Small and isolated populations can exhibit lower genetic diversity, which reduces the ability of individuals to adapt to environmental changes, making them vulnerable to extinction. Thus, estimate the genetic variation of pumas populations that inhabit the Atlantic Forest remnants is essential to assess the current genetic condition of the species and thus provide grants for the creation of strategies for conservation and management. Fecal samples will be collected in protected areas present in the Serra do Mar and Serra da Mantiqueira. The species depositor will be determined through the DNA extraction and amplification of a region of the mitochondrial cytochrome b gene, specific to cats. Subsequently the samples of pumas will be individualized using polymorphic microsatellite loci species-specific. With the panel of multilocus genotypes of all sampled individuals will be made estimates of population density, diversity and genetic relatedness in each of the protected areas, and assessment of genetic differentiation between them. The molecular sexing will be performed through the amplification of a portion of the amelogenin gene, aiming to verify the sex ratio in each Conservation Units.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NAGY-REIS, MARIANA; OSHIMA, JULIA EMI DE FARIA; KANDA, CLAUDIA ZUKERAN; PALMEIRA, FRANCESCA BELEM LOPES; DE MELO, FABIANO RODRIGUES; MORATO, RONALDO GONCALVES; BONJORNE, LILIAN; MAGIOLI, MARCELO; LEUCHTENBERGER, CAROLINE; ROHE, FABIO; et al. NEOTROPICAL CARNIVORES: a data set on carnivore distribution in the Neotropics. ECOLOGY, v. 101, n. 11, NOV 2020. (14/01986-0, 12/14245-2, 10/05343-5, 13/50421-2, 06/04878-7, 17/21816-0, 05/00405-4, 13/24453-4, 15/22844-1, 13/07162-6, 15/19439-8, 16/11595-3, 19/04851-1, 15/18381-6, 13/04957-8, 14/23095-0, 14/09300-0, 11/06782-5, 15/17739-4, 11/22449-4, 14/23132-2, 14/10192-7, 12/00534-2, 14/14925-9)

MORAES DE CASTRO SOUZA, ANDIARA SILOS; SARANHOLI, BRUNO HENRIQUE; CRAWSHAW, JR., PETER GRANSDEN; JAVIER PAVIOLO, AGUSTIN; RAMPIM, LILIAN ELAINE; SARTORELLO, LEONARDO; GALETTI, JR., PEDRO MANOEL. Redescobrindo a onça-pintada em um remanescente de Mata Atlântica costeira no sudeste do Brasil por análise não-invasiva de DNA. Biota Neotropica, v. 17, n. 2, 2017. (12/00534-2, 13/24453-4, 10/52315-7)

DE OLIVEIRA, MARINA E.; SARANHOLI, BRUNO H.; MIOTTO, RENATA A.; SOUZA, ANDIARA SILOS M. C.; DIRZO, RODOLFO; GALETTI JR, PEDRO M.. Female philopatry and unsuccessful male dispersal of a top predator in a human-modified landscape revealed by relatedness analysis. EUROPEAN JOURNAL OF WILDLIFE RESEARCH, v. 69, n. 5, p. 10-pg., 2023-10-01. (22/01741-3, 10/52315-7, 13/24453-4, 11/13897-3, 12/00534-2)