Utilization of cassava waste water dehydrated and whey as culture medium to production of biossurfactant and buteno-2,3-diol by Bacillus subtilis.

• Grant number: 11/22776-5
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: April 01, 2012
• End date: February 29, 2016
• Field of knowledge: Biological Sciences - Biochemistry - Biochemistry of Microorganisms
• Principal Investigator: Glaucia Maria Pastore
• Grantee: Cristiano José de Andrade
• Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 12/20982-0 - ULTRAFILTRANTION IN TWO STEPS OF SURFACTIN PRODUCED BY BACILLUS Subtilis LB5a USING LHYOPHILZED CASSAVA WASTEWATER AS SUBSTRATE, BE.EP.DR

Abstract

The lipopeptide produced by Bacillus subtilis are one of the most studied biosurfactants. Within this group is surfactin, which is one of the most effective agents for surface activity. This compound retains its properties even when subjected to extremes of temperature pH and salinity, and shows several biological activities. The application of industrial waste as a substrate for biosurfactant production by Bacillus subtilis has been studied. The results presented in fermentations in which cassava waste water is one of the culture medium, which has promising results, when considering the results of reduction of surface tension of aqueous solutions and productivity obtained in the bioreactor and pilot-scale. Butane-2,3-diol is a compound which has a promising production by fermentation. It has industrial applications such as monomer for the synthesis of rubber, perfumes, fumigants, moisturizing and softening agents, explosives, plasticizers, food additives, etc. Moreover, the major genus of microrganisms described in the literature associated with butane-2,3-diol are Klebsiella and Bacillus, although the second one, generally, has lower income compared to the first, its use may be favorable due to exclusive synthesis of the isomer D-(-)-butane-2 ,3-diol. The biggest challenge for the implementation of a biotechnological process is generally the economic viability, which is represented mostly by means of culture and purification processes (dow-stream). Therefore, the use of agroindustrial wastes and the concomitant production of two or more products helps reduce the production cost. Among the advantages of drying the substrate, is the smallest volume of material to be stored, the greater self-life, portability, etc. It also allowed the biotechnological industry be installed in different location, than the place which provide the substrate. For the development of this project will be analyzed the physico-chemical of effluents, biosurfactant production and butane-2,3-diol and the kinetics of microbial growth.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ANDRADE, Cristiano José de. Ultrafiltração da surfactina e manosileritritol lipídeos produzidos com manipueira como substrato. 2016. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia de Alimentos Campinas, SP.