FUNCTIONAL STUDY OF DCC AND TGFBR2 GENES IN MULTIPLE MYELOMA CELL LINES

• Grant number: 12/01597-8
• Support Opportunities: Scholarships in Brazil - Master
• Start date: June 01, 2012
• End date: February 28, 2014
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Joel Machado Junior
• Grantee: Dorival Mendes Rodrigues Junior
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil

Abstract

Multiple myeloma (MM) is a B cells malignancy, described as incurable, characterized by multiple organ failure as result of bone marrow infiltration by malignant cells and systemic damage circulating monoclonal protein. Despite considerable progress in understanding the biology of MM, the molecular basis of this disease remains open to debate. The identification of new molecular markers can help to determine prognosis of cancer patients and choose the most appropriate therapy, thus contributing to improved survival rates of these patients. Previous data showed that the hypermethylation of genes DCC or TGFBR2 is significantly correlated with poor prognosis of patients affected with MM. However, as far as we investigated the involvement of proteins encoded by these genes in the tumorigenesis process of MM remains unclear. Therefore, this study aims to better understand the role of these proteins in this disease by silencing (RNAi - shRNA) or overexpression (pcDNATM3.1) of these genes in MM cell lines (RPMI-8226, SK-MM2, SKO-007 and U266) and thus elucidate the contribution of these proteins in different cellular aspects, such as, cell proliferation, adhesion, invasion, cell cycle and apoptosis. Thus, these data can provide direct evidence of the role these genes play in the biology of MM and, moreover, suggest that therapies directed at these proteins may affect the subset of patients with poor prognosis of this disease.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RODRIGUES-JUNIOR, DORIVAL MENDES; BIASSI, THAIS PRISCILA; DE ALBUQUERQUE, GABRIELA ESTRELA; CARLIN, VIVIANE; BURI, MARCUS VINICIUS; MACHADO-JUNIOR, JOEL; VETTORE, ANDRE LUIZ. Downregulation of DCC sensitizes multiple myeloma cells to bortezomib treatment. MOLECULAR MEDICINE REPORTS, v. 19, n. 6, p. 5023-5029, JUN 2019. (12/01597-8, 12/14837-7, 10/20218-2)

RODRIGUES-JUNIOR, DORIVAL MENDES; BIASSI, THAIS PRISCILA; CARLIN, VIVIANE; BURI, MARCUS VINICIUS; TORRECILHAS, ANA CLAUDIA; BORTOLUCI, KARINA RAMALHO; VETTORE, ANDRE LUIZ. OIP5 Expression Sensitize Glioblastoma Cells to Lomustine Treatment. JOURNAL OF MOLECULAR NEUROSCIENCE, v. 66, n. 3, p. 383-389, NOV 2018. (11/15118-1, 10/20218-2, 12/01597-8, 12/14837-7)