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Autophagocytosis and oxidative stress in the central nervous system of aged rats submitted to moderate physical exercise

Grant number: 11/15283-2
Support type:Scholarships in Brazil - Master
Effective date (Start): May 01, 2012
Effective date (End): May 31, 2014
Field of knowledge:Health Sciences - Physical Education
Principal Investigator:Merari de Fátima Ramires Ferrari
Grantee:Carolliny Moura da Silva
Home Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated scholarship(s):13/08063-1 - Analysis of LC3 protein expression in the central nervous system of aged rats submitted to moderate physical exercise, BE.EP.MS

Abstract

The ageing process of central nervous system is accompanied by alterations in cell structure and biochemical composition associated to increase in free radicals and deposit of senile plaques and neurofibrilar tangles. The presence of these aggregates distributed throughout the central nervous system impairs the intracellular environment culminating with cell death. The weakness of cellular pathways of degradation can favor the aggregation of intra- and extracellular proteins, which interferes with neuronal homeostasis and collaborates with neurodegeneration. In view of this, the present study aims to quantify protein carbonylation (as an indirect measurement of oxidative stress) as well as the expression of proteins involved in autophagy system in aged rats submitted to moderate physical exercise and exposed to rotenone (substance that increases oxidative stress and protein aggregation). This study was designed to evaluate the correlation among physical exercise, ageing, oxidative stress and protein aggregation in the central nervous system. Western Blot technique will be employed to detect Ulk1, LC3-I, LC3-II and Beclin-1, which are involved in autophagocytosis, as well as carbonylated proteins. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MELO, KARLA P.; SILVA, CAROLLINY M.; ALMEIDA, MICHAEL F.; CHAVES, RODRIGO S.; MARCOURAKIS, TANIA; CARDOSO, SANDRA M.; DEMASI, MARILENE; NETTO, LUIS E. S.; FERRARI, MERARI F. R. Mild Exercise Differently Affects Proteostasis and Oxidative Stress on Motor Areas During Neurodegeneration: A Comparative Study of Three Treadmill Running Protocols. NEUROTOXICITY RESEARCH, v. 35, n. 2, p. 410-420, FEB 2019. Web of Science Citations: 1.

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