Effects of moderate physical exercise upon intracellular trafficking of neurotroph...
| Grant number: | 11/15283-2 |
| Support type: | Scholarships in Brazil - Master |
| Effective date (Start): | May 01, 2012 |
| Effective date (End): | May 31, 2014 |
| Field of knowledge: | Health Sciences - Physical Education |
| Principal Investigator: | Merari de Fátima Ramires Ferrari |
| Grantee: | Carolliny Moura da Silva |
| Home Institution: | Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
| Associated scholarship(s): | 13/08063-1 - Analysis of LC3 protein expression in the central nervous system of aged rats submitted to moderate physical exercise, BE.EP.MS |
Abstract The ageing process of central nervous system is accompanied by alterations in cell structure and biochemical composition associated to increase in free radicals and deposit of senile plaques and neurofibrilar tangles. The presence of these aggregates distributed throughout the central nervous system impairs the intracellular environment culminating with cell death. The weakness of cellular pathways of degradation can favor the aggregation of intra- and extracellular proteins, which interferes with neuronal homeostasis and collaborates with neurodegeneration. In view of this, the present study aims to quantify protein carbonylation (as an indirect measurement of oxidative stress) as well as the expression of proteins involved in autophagy system in aged rats submitted to moderate physical exercise and exposed to rotenone (substance that increases oxidative stress and protein aggregation). This study was designed to evaluate the correlation among physical exercise, ageing, oxidative stress and protein aggregation in the central nervous system. Western Blot technique will be employed to detect Ulk1, LC3-I, LC3-II and Beclin-1, which are involved in autophagocytosis, as well as carbonylated proteins. (AU) | |