|Support type:||Scholarships in Brazil - Doctorate|
|Effective date (Start):||June 01, 2012|
|Effective date (End):||February 28, 2015|
|Field of knowledge:||Biological Sciences - Microbiology - Biology and Physiology of Microorganisms|
|Principal Investigator:||Eduardo Bagagli|
|Grantee:||Thales Domingos Arantes|
|Home Institution:||Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil|
The soil is the probable habitat of the pathogenic fungus Paracoccidioides brasiliensis, due to its molecular detection in such environmental sample, which may be associated to the high frequency of infection of rural workers as well as wild animals (Dasypus novencinctus, Cabassous centralis and Choloepus didactylus). In the last few years, the molecular detection showed to be promising for the ecological studies about this pathogen, mainly by PCR and Nested PCR techniques. This project aims to detect and differentiate the species Paracoccidioides brasiliensis (complex S1, PS2 and PS3) and Paracoccidioides lutzii in the environment by performing Nested PCR and TSA-FISH, respectively, in aerosol and soil samples from armadillo's burrows from endemic areas for Paracoccidioidomycosis in the Southeast and Mid-Western regions of Brazil. The hybridization with oligonucleotides probes, known as FISH (Fluorescence in situ Hybridization) can be associated with the TSA (Tyramide Signal Amplification) technique, which amplifies the signal emitted by the fluorescent probe. Besides the environmental detection of Paracoccidioides sp. by FISH-TSA, the occurrence of armadillos infected with P. lutzii in the Brazilian Mid-Western, where this species is prevalent, will be studied for the first time (no armadillo from the area has been evaluated so far). Since the current geographic distribution of isolates from Paracoccidioides genus is based mainly on clinical samples and therefore presents the interference of host migration, we believe that the approach here proposed shows a great potential for a better understanding of the biogeography of Paracoccidioides sp., given that the fungal detection will be carried out directly from the environment.