Effects OF pyrethroids ín DIGESTIVES enzymes OF matrinxã, Brycon amazonicus

• Grant number: 12/03898-5
• Support type: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): July 01, 2012
• Effective date (End): October 31, 2014
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal Investigator: Gilberto Moraes
• Grantee: Ive Marchioni Avilez
• Home Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil

Abstract

Pyrethroid insecticides, as deltamethrin, cipermethrin and lambda-cialothrin, are considered ideal products due to its low toxicity to birds and mammals; nevertheless, some studies have reported pyrethroids toxics effects on non-target organisms, such as fish. In these animals, the toxicity is revealed in hematologic and metabolic alterations, damages in liver and nervous system, oxidative stress induction and genotoxity. Due to the wide use of pyrethroids and theirs toxic effects, and knowing that we can monitor fish health by its digestive ability, we can justify justified the need/the importance of studies concerning the damages to digestive enzymes caused by pyrethroids exposure. As it is known, this digestive ability depends both on the presence and on the proper amount of digestive enzymes. Fish are considered good models in ecotoxicology studies and its monitoring permits comprehension of the threats to the in aquatic environment. The matrinxã, Brycon amazonicus, is a fish species native from the Amazon Basin which has been highlighted in Brasilian pisciculture since it presents good growth rate, great acceptability of the artificial diets and its meat is considered tasty. Thus, the aim of this study is to analyze the effects of pyrethroids insecticides (deltamethrin, cipermethrin and lambda-cialothrin) after 96 hours of exposure to 1/10 of the LC50/96 h on the activity of digestives enzymes of fish's gut. Matrinxã juveniles will be exposed to xenobiotics in "in door" tanks system and after the time of exposure, fish will be anesthetized and euthanized to tissues sampling. Digestive enzymes will be evaluated in total gut (unspecific protease, chymotrypsin, trypsin, lipase e amylase).